You are viewing an incomplete version of our website. Please click to reload the website as full version.

Mouse (Murine) Clusterin ELISA Kit

Recommended Clusterin Product (supplied by: Log in to see )

Antigen
Clusterin (CLU) ELISA Kits
  • 40
  • AI893575
  • APO-J
  • APOJ
  • ApoJ
  • Cli
  • CLI
  • CLU
  • clu
  • CLU1
  • CLU2
  • D14Ucla3
  • DAG
  • GP80
  • gpIII
  • KUB1
  • LOC100136120
  • MGC53055
  • MGC89163
  • NA1/NA2
  • RATTRPM2B
  • SGP-2
  • Sgp-2
  • SGP2
  • Sgp2
  • SP-40
  • SP40
  • Sugp-2
  • TRPM-2
  • Trpm2
  • TRPM2
  • TRPM2B
  • Trpmb
  • xclu
  • zgc:73352
Epitope
AA 22-226, AA 227-448
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
21
19
15
7
6
5
5
5
4
3
3
3
2
1
1
Methode Type
Sandwich ELISA
Detection Range
312-20000 pg/mL
Minimum Detection Limit
312 pg/mL
Application
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1672839
$ 399.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN415738 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cerebrospinal Fluid, Urine, Milk, Cell Culture Supernatant, Biological Fluids 18.75-1200 ng/mL 18.75 ng/mL Log in to see 1
0.32666028 ABIN1114200 Sandwich ELISA Serum, Plasma, Biological Fluids 2.5-160 ng/mL 2.5 ng/mL Log in to see
0.32666028 ABIN2708485 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 312-20000 pg/mL 312 pg/mL Log in to see
0.0012121415 ABIN831400 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012121415 ABIN627642 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 10-250 ng/mL 10 ng/mL Log in to see
0.0012121415 ABIN1569042 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 18.75-1200 ng/mL 18.75 ng/mL Log in to see
0.0012121415 ABIN2748021 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 1.3-320 ng/mL Log in to see
0.0012121415 ABIN510782 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012121415 ABIN2065127 Serum, Plasma Log in to see
0.0012121415 ABIN2042408 Sandwich ELISA Cell Culture Supernatant, Plasma (EDTA – heparin), Serum 2-20000 pg/mL 2 pg/mL Log in to see
0.0012121415 ABIN956107 Plasma, Serum Log in to see
0.0012121415 ABIN2683834 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 312-20000 pg/mL 312 pg/mL Log in to see
0.0012121415 ABIN2683836 Sandwich ELISA Serum, Urine 4-128 ng/mL 4 ng/mL Log in to see
0.0012121415 ABIN2762122 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 1.563-100 ng/mL 1.563 ng/mL Log in to see
0.0012121415 ABIN1126854 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012121415 ABIN2683835 Sandwich ELISA Plasma, Serum Log in to see
0.0012121415 ABIN2543059 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cerebrospinal Fluid, Urine, Milk, Cell Culture Supernatant, Biological Fluids 18.75-1200 ng/mL 18.75 ng/mL Log in to see
0.0012121415 ABIN2946417 2.5-160 ng/mL 2.5 ng/mL Log in to see
0.0012121415 ABIN2074792 Sandwich ELISA Log in to see

Top referenced Clusterin ELISA Kit for ELISA

Similar Clusterin ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 20 ELISA Kits

General

Antigen Clusterin (CLU) ELISA Kits
Epitope AA 22-226, AA 227-448
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(21), (19), (15), (7), (6), (5), (5), (5), (4), (3), (3), (3), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 312-20000 pg/mL
Minimum Detection Limit 312 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details Clusterin ELISA Kit

Target details Application Details Handling References for Clusterin Kit (ABIN1672839) Images
Purpose For quantitative detection of Mouse Clusterin in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse Clusterin
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse Clusterin antibod - 1
  • Lyophilized recombinant mouse Clusterin standar - 20ng/tubex2
  • Biotinylated anti- mouse Clusterin antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: D22-R226(beta)&S227-E448(alpha)

Target details

Product Details Clusterin ELISA Kit Application Details Handling References for Clusterin Kit (ABIN1672839) Images back to top
Antigen
Alternative Name CLU (CLU ELISA Kit Abstract)
Background Clusterin(apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
Gene ID 12759
UniProt Q06890
Pathways Apoptosis

Application Details

Product Details Clusterin ELISA Kit Target details Handling References for Clusterin Kit (ABIN1672839) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse Clusterin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Clusterin has been precoated onto 96-well plates. Standards(NSO, D22-R226(β)&S227-E448(α)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Clusterin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Clusterin amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse Clusterin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Clusterin standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.32, Standard deviation: 0.086, CV(%): 6.5
  • Sample 2: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.275, CV(%): 4.1
  • Sample 3: n=16, Mean(ng/ml): 13.5, Standard deviation: 0.567, CV(%): 4.2,
  • Sample 1: n=24, Mean(ng/ml): 1.43, Standard deviation: 0.12, CV(%): 8.4
  • Sample 2: n=24, Mean(ng/ml): 7.4, Standard deviation: 0.555, CV(%): 7.5
  • Sample 3: n=24, Mean(ng/ml): 14.1, Standard deviation: 1.114, CV(%): 7.9
Restrictions For Research Use only

Handling

Product Details Clusterin ELISA Kit Target details Application Details References for Clusterin Kit (ABIN1672839) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Clusterin Kit (ABIN1672839)

Product Details Clusterin ELISA Kit Target details Application Details Handling Images back to top
Background publications

Han, DeMattos, Dugan, Kim-Han, Brendza, Fryer, Kierson, Cirrito, Quick, Harmony, Aronow, Holtzman: "Clusterin contributes to caspase-3-independent brain injury following neonatal hypoxia-ischemia." in: Nature medicine, Vol. 7, Issue 3, pp. 338-43, 2001

Images

Product Details Clusterin ELISA Kit Target details Application Details Handling References for Clusterin Kit (ABIN1672839) back to top
Supplier Images
ELISA image for Clusterin ELISA Kit (CLU) (ABIN1672839) Mouse Clusterin PicoKine ELISA Kit standard curve