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anti-Human Cyclin A1 antibody for Western Blotting

Recommended Cyclin A1 Antibody (supplied by: Log in to see )

Antigen
Cyclin A1 (CCNA1) Antibodies
  • fd14f08
  • wu:fd14f08
  • wu:fi20d12
  • zgc:111869
  • MGC81965
  • cyclin-A1
  • CCNA1
  • MGC132235
  • cyclin-A1-2
  • Cyclin-A1
  • ccna1
  • cyclin-A1-like
  • cyclin A1
  • cyclin-A1-2
  • LOC100147188
  • ccna1
  • CCNA1
  • CYCA1;2
  • Ccna1
  • LOC397885
Epitope
AA 46-93
18
15
10
8
7
6
4
3
3
2
2
1
1
1
Reactivity
Human
78
66
42
34
4
3
3
3
3
3
Host
Mouse
87
50
Clonality
Monoclonal
Conjugate
This Cyclin A1 antibody is un-conjugated
5
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
113
49
27
13
13
11
5
4
3
2
1
1
1
Options
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Catalog No. ABIN967557
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0977407 ABIN714026 IF (p) IHC (p) WB Rabbit IgG AA 250-300 Log in to see Polyclonal 3
3.0977407 ABIN315428 ICC IF IHC (p) IP IHC ELISA WB Rabbit Log in to see Polyclonal 1
3.0977407 ABIN3184139 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.0977407 ABIN1533248 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN3043480 WB Rabbit IgG AA 64-263 Log in to see Polyclonal
3.0977407 ABIN2704688 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.0977407 ABIN1997961 IF ELISA WB Mouse IgG2a AA 1-465 Log in to see 3F6B2C2
3.0977407 ABIN1876832 IHC WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN2163355 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN2163354 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN3029781 WB Rabbit IgG AA 64-263 Log in to see Polyclonal
3.0977407 ABIN948749 WB Mouse AA 1-464, full length Log in to see Polyclonal
3.0977407 ABIN1848494 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN265378 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2878894 IHC (p) ELISA WB Rabbit IgG AA 411-460 Log in to see Polyclonal
1 ABIN790825 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1584312 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2470210 IF IP IHC ELISA WB Rabbit full length Log in to see Polyclonal
1 ABIN1732738 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN714035 IHC (p) WB HRP Rabbit IgG AA 250-300 Log in to see Polyclonal

Top referenced anti-Cyclin A1 antibody for Western Blotting

Similar anti-Cyclin A1 Antibodies

Application / Reactivity Human
ELISA 35 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 27 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 58 Antibodies

General

Antigen Cyclin A1 (CCNA1) Antibodies
Epitope AA 46-93
(18), (15), (10), (8), (7), (6), (4), (3), (3), (2), (2), (1), (1), (1)
Reactivity Human
(78), (66), (42), (34), (4), (3), (3), (3), (3), (3)
Host Mouse
(87), (50)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin A1 antibody is un-conjugated
(5), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(113), (49), (27), (13), (13), (11), (5), (4), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Cyclin A1 Antibody

Target Details Cyclin A1 Application Details Handling References for anti-Cyclin A1 Antibody (ABIN967557) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human cyclin A1 aa. 46-93
Clone B88-2
Isotype IgG1, kappa

Target Details Cyclin A1

Product Details anti-Cyclin A1 Antibody Application Details Handling References for anti-Cyclin A1 Antibody (ABIN967557) Images back to top
Antigen
Alternative Name Cyclin A1 (CCNA1 Antibody Abstract)
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, which allows their binding to cdks to form active complexes that regulate the progression of the cell cycle. These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27[Kip1]. Specific substrates for cyclin-cdk complexes include nuclear lamins, histones, tumor suppressor genes, nucleolin and others. Cyclin A plays a regulatory role during both S-phase and G2/M transitions through its association with cdk2 and cdk1, respectively. A related cyclin, designated cyclin A1, has also been identified. Cyclin A1 shares 48% sequence identity with cyclin A. Yeast two hybrid analysis has shown that, like cyclin A, cyclin A1 also forms a complex with cdk2. Cyclin A1 does not appear to bind cdk1, cdk2 or cdk5. In contrast to cyclin A, cyclin A1 appears to be predominantly expressed in myeloid leukemia cells, however mRNA analysis has suggested that low levels of gene expression may be found in many other hematopoietic and non-hematopoietic cell types. Human cyclin A1 is observed at ~ 65 kD by SDS-PAGE. B88-2 reacts with human cyclin A1. It does not cross-react with cyclin A. A polypeptide fragment containing amino acids 46-93 of human cyclin A1 was used as immunogen.
Molecular Weight 65 kDa
Research Area Cancer, Cell Cycle
Pathways Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication

Application Details

Product Details anti-Cyclin A1 Antibody Target Details Cyclin A1 Handling References for anti-Cyclin A1 Antibody (ABIN967557) Images back to top
Application Notes Clone B88-2 may be used for western blot analysis (8 µg/ml). THP-1 human monocyte cells (ATCC TIB202) are suggested as a positive control for this application.
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Cyclin A1 Antibody Target Details Cyclin A1 Application Details References for anti-Cyclin A1 Antibody (ABIN967557) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Cyclin A1 Antibody (ABIN967557)

Product Details anti-Cyclin A1 Antibody Target Details Cyclin A1 Application Details Handling Images back to top
Product cited in:

Yang, Morosetti, Koeffler: "Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines." in: Cancer research, Vol. 57, Issue 5, pp. 913-20, 1997

Gong, Bhatia, Traganos, Darzynkiewicz: "Expression of cyclins A, D2 and D3 in individual normal mitogen stimulated lymphocytes and in MOLT-4 leukemic cells analyzed by multiparameter flow cytometry." in: Leukemia, Vol. 9, Issue 5, pp. 893-9, 1995

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, 1993

Images

Product Details anti-Cyclin A1 Antibody Target Details Cyclin A1 Application Details Handling References for anti-Cyclin A1 Antibody (ABIN967557) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin A1 antibody (CCNA1) (AA 46-93) (ABIN967557) Western blot analysis of cyclin A1 in THP-1 human monocyte lysate. Anti-human cycllin...
 image for anti-Cyclin A1 antibody (CCNA1) (AA 46-93) (ABIN967557) anti-Cyclin A1 (CCNA1) (AA 46-93) antibody (Image 2)