|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||17 Antibodies||32 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||4 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||11 Antibodies||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||28 Antibodies|
|Antigen||Cyclin A1 (CCNA1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cyclin A1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Cyclin A1 AntibodyTarget Details Cyclin A1 Application Details Handling Images
|Specificity||The antibody detects endogenous levels of Cyclin A1 protein(region surrounding Arg436).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details Cyclin A1Product Details anti-Cyclin A1 Antibody Application Details Handling Images back to top
|Alternative Name||Cyclin A1 (CCNA1 Antibody Abstract)|
|Background||Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.Synonyms: CCNA1, Cyclin-A1|
|Molecular Weight||approx. 52 kDa|
|Research Area||Cancer, Cell Cycle|
|Pathways||Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA|
Application DetailsProduct Details anti-Cyclin A1 Antibody Target Details Cyclin A1 Handling Images back to top
ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cyclin A1 Antibody Target Details Cyclin A1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Cyclin A1 Antibody Target Details Cyclin A1 Application Details Handling back to top