We are preparing the requested document. Please wait, this may take a while...!
|Antigen||DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies|
|Conjugate||This DFFA antibody is un-conjugated Alternatives|
Dot Blot (DB)
|Supplier||Log in to see|
Product Details anti-DFFA AntibodyTarget Details DFFA Application Details Handling Images
|Cross-Reactivity (Details)||Other species not studied.|
|Purification||Protein G affinity chromatography|
|Immunogen||DFFA antibody was raised in mouse using recombinant Human Dna Fragmentation Factor, 45 Kda, Alpha Polypeptide (Dffa)|
Target Details DFFAProduct Details anti-DFFA Antibody Application Details Handling Images back to top
|Alternative Name||DFFA (DFFA Antibody Abstract)|
|Background||Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40 kDa (DFFB) and 45 kDa (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Synonyms: Monoclonal DFFA antibody, Anti-DFFA antibody, DNA fragmentation factor alpha polypeptide antibody, DFF1 antibody, ICAD antibody, DFF-45 antibody.|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-DFFA Antibody Target Details DFFA Handling Images back to top
|Application Notes||Optimal conditions should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-DFFA Antibody Target Details DFFA Application Details Images back to top
|Buffer||DFFA antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).|
|Precaution of Use||This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
|Storage Comment||Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.|
ImagesProduct Details anti-DFFA Antibody Target Details DFFA Application Details Handling back to top