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anti-Human DFFA antibody for Dot Blot

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • A330085O09Rik
  • DFF-45
  • DFF1
  • DFF35
  • Dff45
  • DFFA
  • ICAD
  • ICAD-L
  • ICAD-S
Reactivity
Human
157
73
31
1
1
Host
Mouse
185
31
2
Clonality
Monoclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB)
179
73
40
24
24
20
11
11
6
4
3
3
2
1
1
1
Supplier
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Available images

Catalog No. ABIN931210
$ 285.71
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008906253 ABIN1832162 DB Mouse IgG1 Log in to see 2262C2a
0.0008906253 ABIN3073209 DB WB Mouse IgG1 Log in to see 8J120
0.0008906253 ABIN2598325 DB WB Mouse IgG1 Log in to see 2262C2a

Similar anti-DFFA Antibodies

Application / Reactivity Human
Dot Blot (DB) 4 Antibodies
ELISA 63 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 20 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 133 Antibodies

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Reactivity Human
(157), (73), (31), (1), (1)
Host Mouse
(185), (31), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Dot Blot (DB)
(179), (73), (40), (24), (24), (20), (11), (11), (6), (4), (3), (3), (2), (1), (1), (1)
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling Images
Cross-Reactivity Human
Cross-Reactivity (Details) Other species not studied.
Purification Protein G affinity chromatography
Immunogen DFFA antibody was raised in mouse using recombinant Human Dna Fragmentation Factor, 45 Kda, Alpha Polypeptide (Dffa)
Clone 2262C2a
Isotype IgG1

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFFA (DFFA Antibody Abstract)
Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40 kDa (DFFB) and 45 kDa (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Synonyms: Monoclonal DFFA antibody, Anti-DFFA antibody, DNA fragmentation factor alpha polypeptide antibody, DFF1 antibody, ICAD antibody, DFF-45 antibody.
Research Area DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling Images back to top
Application Notes Optimal conditions should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details Images back to top
Concentration 100 µg/mL
Buffer DFFA antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage 4 °C
Storage Comment Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (ABIN931210) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody