anti-Human DFFA antibody for Immunohistochemistry (Frozen Sections)

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
C-Term
28
26
19
16
15
15
12
8
6
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
166
85
41
2
2
1
1
1
Host
Rabbit
192
35
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
192
71
48
36
33
28
19
13
6
4
4
3
2
1
1
Supplier
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Catalog No. ABIN318989
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN187713 IHC (fro) WB Rabbit AA 2-21 Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope C-Term
(28), (26), (19), (16), (15), (15), (12), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(166), (85), (41), (2), (2), (1), (1), (1)
Host Rabbit
(192), (35), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(192), (71), (48), (36), (33), (28), (19), (13), (6), (4), (4), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN318989) Images
Specificity This antibody recognises the 45 kDa chain of a heterodimeric apoptosis related protein designated DFF (DNA fragmentation factor). DFF-45 is a substrate for Caspase-3, which cleaves the protein at two sites to generate factors that cause DNA fragmentation. This antibody recognises both intact DFF-45 and cleaved fragments at the C-terminus.
Purification Purified
Immunogen Peptide from C-terminus of human DFF
Isotype IgG

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling References for anti-DFFA antibody (ABIN318989) Images back to top
Antigen
Alternative Name DFFA / ICAD (DFFA Antibody Abstract)
Background Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Gene ID 1676
NCBI Accession NP_004392
UniProt O00273
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA antibody (ABIN318989) Images back to top
Application Notes Immunohistochemistry on frozen sections. Western Blot: 1/1000 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA antibody (ABIN318989) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DFFA antibody (ABIN318989)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Background publications

Liu, Zou, Slaughter, Wang: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN318989) back to top
Supplier Images
 image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibody (ABIN318989) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibody
 image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibody (ABIN318989) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibody (Image 2)