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anti-Human DFFA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
AA 2-21
37
28
17
16
13
13
11
9
5
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
165
77
31
1
1
Host
Rabbit
194
33
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
188
76
47
32
32
21
18
11
6
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN615234
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.229811 ABIN461876 IHC (p) WB Rabbit AA 2-21 Log in to see Polyclonal
11.229811 ABIN4305092 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal
11.229811 ABIN4305088 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
11.229811 ABIN4305089 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
11.229811 ABIN4305082 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN272297 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4305087 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
1 ABIN1687985 IHC (p) Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1687962 IHC (p) Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2889986 IHC (p) ELISA WB Rabbit IgG AA 151-200 Log in to see Polyclonal
1 ABIN373373 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN303253 IF IHC (p) IP WB Rabbit AA 313-331 Log in to see Polyclonal
1 ABIN2891161 IHC (p) ELISA Rabbit IgG AA 175-224 Log in to see Polyclonal
1 ABIN1733624 ICC IHC (p) IP WB Rabbit IgG AA 313-331 Log in to see Polyclonal
1 ABIN214483 ICC IHC (p) IP WB Rabbit AA 313-331 Log in to see Polyclonal
1 ABIN1733623 IHC (p) WB Rabbit IgG AA 2-21 Log in to see Polyclonal
1 ABIN2119453 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 51-305 Log in to see Polyclonal
1 ABIN4305090 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN1978399 IF IP IHC (p) WB Rabbit IgG AA 271-331 Log in to see Polyclonal
1 ABIN1978400 IF IHC (p) Rabbit IgG AA 271-331 Log in to see Polyclonal

Top referenced anti-DFFA antibody for Immunohistochemistry (Paraffin-embedded Sections)

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General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope AA 2-21
(37), (28), (17), (16), (13), (13), (11), (9), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(165), (77), (31), (1), (1)
Host Rabbit
(194), (33), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(188), (76), (47), (32), (32), (21), (18), (11), (6), (4), (4), (3), (2), (1), (1), (1)
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling Images
Specificity This antibody reacts to DNA Fragmentation Factor, 45 kDa, Alpha Polypeptide (DFF45/ICAD) (DFFA).
Purification DEAE-column Chromatography
Immunogen DFFA antibody was raised against synthetic peptide

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFFA / ICAD (DFFA Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kD DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Gene ID 1676
NCBI Accession NP_004392
UniProt O00273
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 2-21) (ABIN615234) Human Breast (formalin-fixed, paraffin-embedded) stained with DFFAat 10 µg/ml followe...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 2-21) (ABIN615234) Human Colon (formalin-fixed, paraffin-embedded) stained with DFFAat 10 µg/ml followed...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 2-21) (ABIN615234) Human Pancreas (formalin-fixed, paraffin-embedded) stained with DFFAat 10 µg/ml follo...
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 2-21) (ABIN615234) Western blot analysis of DFF45/35 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) who...