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anti-Human DFFA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
AA 2-21
31
28
20
15
15
15
14
9
5
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
166
87
41
2
1
Host
Rabbit
195
35
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
195
75
48
33
32
27
18
13
6
4
4
3
2
2
1
1
Supplier
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Catalog No. ABIN461876
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.697414 ABIN615234 IHC (p) WB Rabbit AA 2-21 Log in to see Polyclonal
12.697414 ABIN4305089 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
12.697414 ABIN4305092 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal
12.697414 ABIN4305088 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
12.697414 ABIN4305082 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4305087 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN272297 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1687985 IHC (p) Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1687962 IHC (p) Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN303253 IF IHC (p) IP WB Rabbit AA 313-331 Log in to see Polyclonal
1 ABIN373374 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN214483 ICC IHC (p) IP WB Rabbit AA 313-331 Log in to see Polyclonal
1 ABIN2889986 IHC (p) ELISA WB Rabbit IgG AA 151-200 Log in to see Polyclonal
1 ABIN2891161 IHC (p) ELISA Rabbit IgG AA 175-224, Cleaved-Asp224 Log in to see Polyclonal
1 ABIN4305090 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN1733624 ICC IHC (p) IP WB Rabbit IgG AA 313-331 Log in to see Polyclonal
1 ABIN1733623 IHC (p) WB Rabbit IgG AA 2-21 Log in to see Polyclonal
1 ABIN2892223 IF IHC (p) WB Rabbit Gln177 Log in to see Polyclonal
1 ABIN2598329 ICC IF IHC (p) IP WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN1978400 IF IHC (p) Rabbit IgG AA 271-331 Log in to see Polyclonal

Top referenced anti-DFFA antibody for Immunohistochemistry (Paraffin-embedded Sections)

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General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope AA 2-21
(31), (28), (20), (15), (15), (15), (14), (9), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(166), (87), (41), (2), (1)
Host Rabbit
(195), (35), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(195), (75), (48), (33), (32), (27), (18), (13), (6), (4), (4), (3), (2), (2), (1), (1)
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling Images
Brand IHC-plus™
Specificity Peptide corresponding to amino acids 2 to 21 of human DFF45
Purification Ion exchange chromatography
Immunogen Synthetic peptide from human DFFA / ICAD.

Type of Immunogen: Synthetic peptide

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFFA / ICAD / DFF45 (DFFA Antibody Abstract)
Background Name/Gene ID: DFFA
Family: DNase

Synonyms: DFFA, DFF-45, DFF1, Dna fragmentation factor-45, ICAD, Inhibitor of CAD, DFF45
Gene ID 1676
UniProt O00273
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling Images back to top
Application Notes Approved: IHC-P (10 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles. Store undiluted.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 2-21) (ABIN461876) Human Breast (formalin-fixed, paraffin-embedded) stained with DFFA antibody ABIN46187...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 2-21) (ABIN461876) Human Colon (formalin-fixed, paraffin-embedded) stained with DFFA antibody ABIN461876...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 2-21) (ABIN461876) Human Pancreas (formalin-fixed, paraffin-embedded) stained with DFFA antibody ABIN461...