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anti-Mouse (Murine) DFFA antibody for Enzyme Immunoassay

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • A330085O09Rik
  • DFF-45
  • DFF1
  • DFF35
  • Dff45
  • DFFA
  • ICAD
  • ICAD-L
  • ICAD-S
Epitope
C-Term
37
29
18
16
13
13
11
9
5
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
166
77
32
1
1
Host
Rabbit
194
33
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
187
77
48
32
31
20
18
11
5
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN499958
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN499956 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.0008867729 ABIN272297 EIA IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-DFFA antibody for Enzyme Immunoassay

Similar anti-DFFA Antibodies

Application / Reactivity Mouse (Murine)
ELISA 19 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Western Blotting (WB) 60 Antibodies

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope C-Term
(37), (29), (18), (16), (13), (13), (11), (9), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(166), (77), (32), (1), (1)
Host Rabbit
(194), (33), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(187), (77), (48), (32), (31), (20), (18), (11), (5), (4), (4), (3), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN499958) Images
Specificity ICAD antibody was raised against a peptide corresponding to amino acids 312 to 331 of mouse ICAD .
Purification Affinity chromatography purified via peptide column
Immunogen Mouse ICAD (C-Terminus) Peptide
Isotype IgG

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling References for anti-DFFA Antibody (ABIN499958) Images back to top
Antigen
Alternative Name DFFA / ICAD (DFFA Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Gene ID 13347
UniProt O54786
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA Antibody (ABIN499958) Images back to top
Application Notes ELISA. Western Blot: 1/500 to 1/2000. A 45 kDa band can be detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA Antibody (ABIN499958) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-DFFA Antibody (ABIN499958)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Background publications

Enari, Sakahira, Yokoyama, Okawa, Iwamatsu, Nagata: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998

Wyllie: "Apoptosis. An endonuclease at last." in: Nature, Vol. 391, Issue 6662, pp. 20-1, 1998

Liu, Zou, Slaughter, Wang: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN499958) back to top
Supplier Images
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (C-Term) (ABIN499958) Western blot analysis of ICAD in mouse lung (L), brain (B), liver (L), and kidney tis...