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anti-Human DFFB antibody for Immunofluorescence

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • 40kDa
  • 5730477D02Rik
  • CAD
  • Cad
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Didff
  • LOC100223514
Epitope
N-Term
26
18
13
9
8
5
5
5
4
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Human
101
69
49
1
Host
Rabbit
125
10
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
106
38
25
22
21
18
17
4
3
3
3
1
Supplier
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Catalog No. ABIN499738
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3157438 ABIN499740 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal 5
0.3157438 ABIN499736 EIA IF WB Rabbit IgG Log in to see Polyclonal 5
0.0010826217 ABIN303254 IF IHC (p) WB Rabbit Center Log in to see Polyclonal

Top referenced anti-DFFB antibody for Immunofluorescence

Similar anti-DFFB Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 35 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Western Blotting (WB) 84 Antibodies

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope N-Term
(26), (18), (13), (9), (8), (5), (5), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(101), (69), (49), (1)
Host Rabbit
(125), (10)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(106), (38), (25), (22), (21), (18), (17), (4), (3), (3), (3), (1)
Pubmed 5 references available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN499738) Images
Specificity DFF40 antibody was raised against a peptide corresponding to amino acids 3 to 18 of human DFF40.
Purification Affinity chromatography purified via peptide column
Immunogen Human DFF40 (N-Terminus) Peptide
Isotype IgG

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB Antibody (ABIN499738) Images back to top
Antigen
Alternative Name DFFB / CAD (DFFB Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.Synonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta
Gene ID 1677
NCBI Accession NP_004393
UniProt O76075
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB Antibody (ABIN499738) Images back to top
Application Notes ELISA. Western Blot: 1/500 to 1/2000. A 40 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB Antibody (ABIN499738) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-DFFB Antibody (ABIN499738)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Nagai, Dohmae, Nishimura, Kato, Hirata, Takahashi: "Operando XAFS study of catalytic NO reduction over Cu/CeO2: the effect of copper-ceria interaction under periodic operation." in: Physical chemistry chemical physics : PCCP, Vol. 15, Issue 22, pp. 8461-5, 2013

Enari, Sakahira, Yokoyama, Okawa, Iwamatsu, Nagata: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998

Halenbeck, MacDonald, Roulston, Chen, Conroy, Williams: "CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45." in: Current biology : CB, Vol. 8, Issue 9, pp. 537-40, 1998

Liu, Li, Widlak, Zou, Luo, Garrard, Wang: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN499738) back to top
Supplier Images
Immunofluorescence (IF) image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (N-Term) (ABIN499738) Immunocytochemistry of DFF40 in Jurkat cells with AP30287PU-N DFF40 antibody at 5 μg/ml.
Western Blotting (WB) image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (N-Term) (ABIN499738) Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with AP30...