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anti-Cow (Bovine) DIABLO antibody for Western Blotting

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
C-Term
35
31
13
11
8
6
5
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Pig (Porcine)
169
62
42
4
3
2
1
1
1
1
Host
Rabbit
128
50
9
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
176
78
67
44
30
16
11
9
6
4
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2792127
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN925989 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-DIABLO antibody for Western Blotting

Similar anti-DIABLO Antibodies

Application / Reactivity Human Cow (Bovine) Dog (Canine) Pig (Porcine)
Western Blotting (WB) 160 Antibodies 2 Antibodies 4 Antibodies 3 Antibodies
Neutralization (Neut) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 25 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunohistochemistry (IHC) 57 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunofluorescence (IF) 16 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope C-Term
(35), (31), (13), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Pig (Porcine)
(169), (62), (42), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(128), (50), (9), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(176), (78), (67), (44), (30), (16), (11), (9), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN2792127) Images
Sequence LVKLQVEEVH QLSRKAETKL AEAQIEELRQ KTQEEGEERA ESEQEAYLRE
Predicted Reactivity Human : 100 %, Dog : 90 %, Bovine : 80 %, Horse : 80 %, Pig : 80 %
Characteristics This is a rabbit polyclonal antibody against DIABLO. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human DIABLO

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN2792127) Images back to top
Antigen
Alternative Name DIABLO (DIABLO Antibody Abstract)
Background DIABLO is an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively.
Molecular Weight 21 kDa
Gene ID 56616
NCBI Accession NM_138930, NP_620308
UniProt Q502X2
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN2792127) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 186 AA

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN2792127) Images back to top
Format Liquid
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DIABLO Antibody (ABIN2792127)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Background publications

Samuel, Welsh, Lober, Togo, Zapata, Reed: "Distinct BIR domains of cIAP1 mediate binding to and ubiquitination of tumor necrosis factor receptor-associated factor 2 and second mitochondrial activator of caspases." in: The Journal of biological chemistry, Vol. 281, Issue 2, pp. 1080-90, 2006

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN2792127) back to top
Supplier Images
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (C-Term) (ABIN2792127) WB Suggested Anti-DIABLO Antibody Titration: 0.3125ug/ml
ELISA Titer: