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anti-Human DIABLO antibody for Flow Cytometry

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
AA 56-239
32
15
13
11
8
6
4
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
138
55
35
4
4
3
2
1
1
Host
Rabbit
117
30
8
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is conjugated to FITC
1
Application
Flow Cytometry (FACS)
145
58
57
32
23
11
7
6
4
4
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1996478
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3068567 ABIN2213691 FACS Mouse Log in to see
0.0010666476 ABIN189310 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y12
0.0010666476 ABIN2505678 ICC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 10G7
0.0010666476 ABIN199860 ICC FACS IP ELISA WB Rat IgG2a Log in to see

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Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 50 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 50 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 21 Antibodies
Immunostaining (ISt) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 131 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 56-239
(32), (15), (13), (11), (8), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(138), (55), (35), (4), (4), (3), (2), (1), (1)
Host Rabbit
(117), (30), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is conjugated to FITC
(1)
Application Flow Cytometry (FACS)
(145), (58), (57), (32), (23), (11), (7), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Specificity Human SMAC / Diablo
Characteristics This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo, NP_063940.1, Ala56-Asp239) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Immunogen Recombinant Human SMAC / Diablo protein
Clone 8
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO (DIABLO Antibody Abstract)
Background Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Apoptosis is carried out by caspases which reside in cells as inactive zymogens, and the initiator caspase zymogens are activated by adaptor proteins such as FADD and Apaf-1 mediated either by death receptors or cytochrome c. DIABLO (direct IAP binding protein with low pI), also well known as SMAC (second mitochondria derived activator of caspase), is a mitochondrial homodimeric protein, and performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. It has been shown that DIABLO mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. DIABLO is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs.
NCBI Accession NP_063940.1
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes Concentration: 10 μl/Test, 0.1 mg/ml
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Format Liquid
Buffer Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2 °C-8 °C.
Expiry Date 12 months

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (FITC) (ABIN1996478) Flow cytometric analysis of Human Diablo expression in HeLa cells. The cells were tre...