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anti-Human DIABLO antibody for Flow Cytometry

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Epitope
AA 56-239
36
32
13
11
8
6
4
4
4
3
2
2
2
1
1
1
1
Reactivity
Human
175
71
57
4
4
3
3
3
2
2
1
1
Host
Rabbit
129
50
7
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is conjugated to FITC
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
174
73
65
46
30
19
9
7
6
4
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1996478
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.7700815 ABIN2213691 FACS Mouse Log in to see
1 ABIN4905220 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN199860 ICC FACS IP ELISA WB Rat IgG2a Log in to see

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General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 56-239
(36), (32), (13), (11), (8), (6), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(175), (71), (57), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(129), (50), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is conjugated to FITC
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(174), (73), (65), (46), (30), (19), (9), (7), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Specificity Human SMAC / Diablo
Characteristics This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo, ABIN2002616 NP_063940.1, Ala56-Asp239, and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Immunogen Recombinant Human SMAC / Diablo protein (ABIN2002616)
Clone 008
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO (DIABLO Antibody Abstract)
Background Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Apoptosis is carried out by caspases which reside in cells as inactive zymogens, and the initiator caspase zymogens are activated by adaptor proteins such as FADD and Apaf-1 mediated either by death receptors or cytochrome c. DIABLO (direct IAP binding protein with low pI), also well known as SMAC (second mitochondria derived activator of caspase), is a mitochondrial homodimeric protein, and performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. It has been shown that DIABLO mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. DIABLO is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs.
NCBI Accession NP_063940.1
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Format Liquid
Concentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Expiry Date 12 months

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (FITC) (ABIN1996478) Flow cytometric analysis of Human Diablo expression in HeLa cells. The cells were tre...