anti-Human DIABLO antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Epitope
AA 177-211
39
32
13
11
8
6
4
4
4
3
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
185
70
56
4
4
3
3
3
3
2
1
1
Host
Rabbit
136
53
7
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
183
73
66
49
31
20
9
8
7
4
4
3
2
2
1
1
1
1
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Catalog No. ABIN674619
$ 307.45
Plus shipping costs $45.00

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 177-211
(39), (32), (13), (11), (8), (6), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(185), (70), (56), (4), (4), (3), (3), (3), (3), (2), (1), (1)
Host Rabbit
(136), (53), (7), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(183), (73), (66), (49), (31), (20), (9), (8), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling ProductDetails: References for anti-DIABLO antibody (ABIN674619) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Smac
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling ProductDetails: References for anti-DIABLO antibody (ABIN674619) Images back to top
Antigen
Alternative Name Smac/Diablo (DIABLO Antibody Abstract)
Background

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Subcellular location: Helical, Mitochondrion

Synonyms: SMAC, DFNA64, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, DIABLO

Gene ID 56616
UniProt Q9NR28
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling ProductDetails: References for anti-DIABLO antibody (ABIN674619) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details ProductDetails: References for anti-DIABLO antibody (ABIN674619) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

ProductDetails: References for anti-DIABLO antibody (ABIN674619)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Liu, Chen, Li, Wang, Cheng: "Smac/DIABLO regulates the apoptosis of hypertrophic scar fibroblasts." in: International journal of molecular medicine, Vol. 32, Issue 3, pp. 615-22, 2013

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling ProductDetails: References for anti-DIABLO antibody (ABIN674619) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 177-211) antibody (ABIN674619) Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Smac/DI...
SDS-PAGE (SDS) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 177-211) antibody (ABIN674619) Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti AVPR2 Polyclona...
Immunohistochemistry (IHC) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 177-211) antibody (ABIN674619) Gastric Cancer Cells labeled with Anti-Smac/DIABLO Polyclonal Antibody, Unconjugated ...