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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||16 Antibodies||24 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||6 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||6 Antibodies|
|Immunofluorescence (IF)||6 Antibodies||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||36 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Antigen||Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This DIABLO antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-DIABLO AntibodyTarget Details DIABLO Application Details Handling Images
|Purification||Protein A purified|
|Immunogen||A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.|
Target Details DIABLOProduct Details anti-DIABLO Antibody Application Details Handling Images back to top
|Alternative Name||DIABLO / SMAC (DIABLO Antibody Abstract)|
Smac/Diablo (Second Mitochondria-derived Activator of Caspases/Direct IAP BindingProtein with Low PI) is localized to the mitochondria and involved in activatingprogrammed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/Diablois released from the mitochondria and binds anti-apoptotic IAP proteins, suppressingtheir inhibitory activity and promoting caspase activation (1,2). This interaction involvesprimarily the amino-terminal residues of Smac/Diablo with the BIR3 region of the IAPprotein (3,4). Alternate Names: anti-0610041G12Rik antibody, anti-DBOH antibody, anti-Diablo homolog (Drosophila)antibody, anti-Diablo homolog antibody, anti-Diablo homolog mitochondrial antibody,anti-DIABLO S antibody, anti-Direct IAP binding protein with low pI antibody,anti-FLJ10537 antibody, anti-FLJ25049 antibody, anti-Mitochondrial Smac proteinantibody, anti-Second mitochondria derived activator of caspase antibody, anti-SMAC 3antibody, anti-Smac protein antibody, anti-SMAC3 antibody.
Gene Symbol: DIABLO
|Gene ID||56616, 66593, 288753|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 21/27kDa.
Recommended dilutions: Flow Cytometry 1:10-50, Immunocytochemistry/Immunofluorescence 1:10, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10, Immunoprecipitation 1:10-80, Western Blot 1:1000
Protocol specific for Smac Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top