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anti-Human DIABLO antibody for Western Blotting

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Reactivity
Human, Mouse (Murine), Rat (Rattus)
167
59
39
4
4
3
2
1
1
Host
Rabbit
127
49
9
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
174
78
65
43
28
15
10
9
5
4
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN223042
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN2792127 WB Rabbit C-Term Log in to see Polyclonal 1
0.13476625 ABIN500784 EIA IHC (p) WB Rabbit IgG AA 222-237, C-Term Log in to see Polyclonal 1
0.13476625 ABIN968785 IF WB Mouse IgG2b AA 125-239 Log in to see 56-Smac-DIABLO 4
0.13476625 ABIN393216 WB Mouse IgG2a kappa AA 119-218 Log in to see 4F9 5
0.13476625 ABIN1996479 ELISA WB Rabbit IgG AA 56-239 Log in to see Polyclonal 2
0.13476625 ABIN492563 IHC (p) WB Mouse IgG2b Log in to see 79-1-83
0.13476625 ABIN121596 IHC (p) WB Rabbit IgG AA 222-237 Log in to see Polyclonal
0.13476625 ABIN3044515 IHC (p) ELISA WB Rabbit IgG AA 56-239 Log in to see Polyclonal
0.13476625 ABIN1996471 ELISA WB Mouse IgG1 AA 56-239 Log in to see 7C8A5E6
0.13476625 ABIN189310 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y12
0.13476625 ABIN2463658 ELISA WB Rabbit Log in to see Polyclonal
0.13476625 ABIN1872269 IHC WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN106863 WB Rabbit AA 56-239, His6 Log in to see Polyclonal
0.13476625 ABIN2856427 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.13476625 ABIN1996481 IP ELISA WB Rabbit IgG AA 56-239 Log in to see Polyclonal
0.13476625 ABIN1996475 ELISA WB Rabbit IgG AA 56-239 Log in to see 6
0.13476625 ABIN527932 ELISA WB Mouse IgG2a kappa AA 119-218, partial Log in to see 4F9
0.13476625 ABIN527930 WB Mouse AA 1-239, full length Log in to see Polyclonal
0.13476625 ABIN566031 ELISA WB Mouse IgG2a kappa AA 119-218, partial Log in to see 4F9
0.13476625 ABIN2476526 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 2

Top referenced anti-DIABLO antibody for Western Blotting

Similar anti-DIABLO Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 160 Antibodies 61 Antibodies 41 Antibodies
Neutralization (Neut) 1 Antibodies 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 25 Antibodies 13 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies 21 Antibodies 15 Antibodies
Immunohistochemistry (IHC) 57 Antibodies 38 Antibodies 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 4 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(167), (59), (39), (4), (4), (3), (2), (1), (1)
Host Rabbit
(127), (49), (9), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(174), (78), (65), (43), (28), (15), (10), (9), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN223042) Images
Blocking Peptide Blocking peptide for this product available: ABIN412906
Specificity The immunoaffinity purified antibody detects the 25 kDa Smac/DIABLO in samples from human, mouse, and rat origins.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 220 of mouse Smac/DIABLO
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN223042) Images back to top
Antigen
Alternative Name Smac/DIABLO (DIABLO Antibody Abstract)
Background Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Smac/DIABLO binds to IAPs (Inhibitor of Apoptosis Proteins) and neutralizes the inhibitory effect of IAPs. Analysis of the structural basis of Smac/DIABLO has revealed that the N-terminal amino acids are required for Smac/DIABLO to bind to IAPs and activation of caspases.
Synonyms: SMAC, SMAC3, DIABLO, DIABLO-S , FLJ10537, FLJ25049, 0610041G12Rik
Gene ID 66593
UniProt Q9JIQ3
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN223042) Images back to top
Application Notes Western blotting (0.5-4 μg/mL) and Immunohistochemistry (5-20 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN223042) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-Smac/DIABLO polyclonal antibody in PBS ( pH 7.2) containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-DIABLO Antibody (ABIN223042)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Gesing, Masternak, Wang, Lewinski, Karbownik-Lewinska, Bartke: "Decreased expression level of apoptosis-related genes and/or proteins in skeletal muscles, but not in hearts, of growth hormone receptor knockout mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 236, Issue 2, pp. 156-68, 2011

Haudek, Taffet, Schneider, Mann: "TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2692-701, 2007

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN223042) back to top
Supplier Images
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (ABIN223042) Western blot analysis of Smac expression with human heart tissue lysate.