anti-Monkey DIABLO antibody for Western Blotting

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Reactivity
Human, Monkey
185
71
57
4
3
3
3
3
3
2
1
1
Host
Mouse
137
52
7
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
183
73
66
49
31
20
9
8
7
4
4
3
2
2
1
1
1
1
1
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Catalog No. ABIN492563
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN462354 IHC (p) WB Mouse IgG2b Log in to see 79-1-83
1 ABIN1723258 IHC (p) WB Mouse IgG2b full length Log in to see 79-1-83
1 ABIN2469972 IHC WB Mouse IgG2b Log in to see 79-1-83

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Reactivity Human, Monkey
(185), (71), (57), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Mouse
(137), (52), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(183), (73), (66), (49), (31), (20), (9), (8), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Specificity Recognizes Second Mitochondria-derived Activator of Caspase (SMAC/DIABLO).
Cross-Reactivity (Details) Species reactivity (expected):Monkey.
Species reactivity (tested):Human.
Characteristics Synonyms: Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, DirectIAP-binding protein with low pI
Purification Protein G Chromatograghy.
Immunogen Recombinant Human Smac/DIABLO protein.
Clone 79-1-83
Isotype IgG2b

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background Second mitochondria-derived activator of caspase (SMAC) has been implicated in the activation of apoptosis in response to cell stress. SMAC promotes CASP9 activation by binding to IAPs and removing their inhibitory activity. The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Smac is an antagonist of the X-linked inhibitor of apoptosis (XIAP) - the most potent caspase inhibitor of all known inhibitor of apoptosis-family members. Smac promotes apoptosis by eliminating the inhibitory effect of inhibitor-of-apoptosis (IAPs) through physical interactions.Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 56616
UniProt Q9NR28
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes Western Blot. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This DIABLO/SMAC antibody was validated for use in immunohistochemistry on a panel of21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (ABIN492563) anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody
Immunohistochemistry (IHC) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (ABIN492563) anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (ABIN492563) anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (Image 3)
Western Blotting (WB) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (ABIN492563) anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (Image 4)