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anti-Mouse (Murine) DIABLO antibody for Immunohistochemistry (Frozen Sections)

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Reactivity
Mouse (Murine)
168
59
40
4
4
3
2
1
1
Host
Rat
128
49
8
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
174
78
66
42
27
14
10
9
4
4
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN187401
$ 654.87
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000884117 ABIN4354647 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 55-237 Log in to see 9H10

Similar anti-DIABLO Antibodies

Application / Reactivity Mouse (Murine)
ELISA 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 38 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 61 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Reactivity Mouse (Murine)
(168), (59), (40), (4), (4), (3), (2), (1), (1)
Host Rat
(128), (49), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(174), (78), (66), (42), (27), (14), (10), (9), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Specificity This antibody recognizes mouse Smac/DIABLO. Species Reactivity: Mouse. Others not tested.
Immunogen Recombinant mouse Smac/DIABLO (aa 55 - 237).
Clone 9H10
Isotype IgG2a

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background A novel protein Smac/DIABLO has been identified, which promotes apoptosis by removing MIHA/XIAP from processed caspase-9 in the cytochrome c/Apaf-1/caspase-9 pathway. Smac/DIABLO promotes caspase-9 activation by binding to inhibitor of apoptosis proteins (IAPs), and removing their inhibitory activity. Smac/DIABLO is normally a mitochondrial protein but is released into the cytosol when cells undergo apoptosis. Mitochondrial import and cleavage of its signal peptide are required for Smac/DIABLO to gain its apoptotic activity, which is supported by structural evidence. Overexpression of Smac/DIABLO increases the sensitivity of cells to apoptotic stimuli. Smac/DIABLO is the second mitochondrial protein described, along with cytochrome c, that promotes apoptosis by activating caspases. Smac/DIABLO is the first mammalian protein to be identified that directly inhibits IAP function.
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes ELISA Flow cytometry Immunocytochemistry Immunohistochemistry: Frozen & paraffin sections. Immunoprecipitation Western blot The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Format Liquid
Buffer 100 µg of monoclonal antibody at 1 mg/mL in PBS containing 0.02% sodium azide, concentration of 1 mg/mL. 95% purity as determined by SDS-PAGE. The antibody was purified by Protein G affinity chromatography.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C