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anti-Mouse (Murine) DIABLO antibody for Western Blotting

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Reactivity
Human, Mouse (Murine), Rat (Rattus)
175
70
56
4
4
3
3
3
2
2
1
1
Host
Rabbit
129
50
7
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
173
73
64
46
30
19
9
8
6
4
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN223042
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0741942 ABIN500784 EIA IHC (p) WB Rabbit IgG AA 222-237, C-Term Log in to see Polyclonal 1
3.0741942 ABIN153019 ICC IF IP WB Mouse IgG3, IgG1 Log in to see 78-1-118 9
3.0741942 ABIN4354647 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 55-237 Log in to see 9H10 3
3.0741942 ABIN4354645 ELISA ICC IF IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 1
3.0741942 ABIN3044515 IHC (p) ELISA WB Rabbit IgG AA 56-239 Log in to see Polyclonal
3.0741942 ABIN121596 IHC (p) WB Rabbit IgG AA 222-237 Log in to see Polyclonal
3.0741942 ABIN4354640 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
3.0741942 ABIN1872269 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0741942 ABIN2856427 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.0741942 ABIN3198321 IC IF WB Rabbit Log in to see Polyclonal
3.0741942 ABIN4354644 ELISA IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
3.0741942 ABIN2476526 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 2
3.0741942 ABIN252164 IHC IHC (fro) IHC (p) IP SimWes WB Rabbit Log in to see Polyclonal
3.0741942 ABIN2428719 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0741942 ABIN2422157 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0741942 ABIN2405470 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0741942 ABIN1030687 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
3.0741942 ABIN1030686 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN674619 IF (p) IHC (p) WB Rabbit IgG AA 177-211 Log in to see Polyclonal 1
1 ABIN121595 IHC (p) WB Rabbit IgG AA 225-239 Log in to see Polyclonal

Top referenced anti-DIABLO antibody for Western Blotting

Similar anti-DIABLO Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 1 Antibodies 1 Antibodies
Simple Western (SimWes) 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 162 Antibodies 69 Antibodies 55 Antibodies
Neutralization (Neut) 1 Antibodies 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 25 Antibodies 18 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies 25 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(175), (70), (56), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(129), (50), (7), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(173), (73), (64), (46), (30), (19), (9), (8), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN223042) Images
Blocking Peptide Blocking peptide for this product available: ABIN412906
Specificity The immunoaffinity purified antibody detects the 25 kDa Smac/DIABLO in samples from human, mouse, and rat origins.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 220 of mouse Smac/DIABLO
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN223042) Images back to top
Antigen
Alternative Name Smac/DIABLO (DIABLO Antibody Abstract)
Background Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Smac/DIABLO binds to IAPs (Inhibitor of Apoptosis Proteins) and neutralizes the inhibitory effect of IAPs. Analysis of the structural basis of Smac/DIABLO has revealed that the N-terminal amino acids are required for Smac/DIABLO to bind to IAPs and activation of caspases.
Synonyms: SMAC, SMAC3, DIABLO, DIABLO-S , FLJ10537, FLJ25049, 0610041G12Rik
Gene ID 66593
UniProt Q9JIQ3
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN223042) Images back to top
Application Notes Western blotting (0.5-4 μg/mL) and Immunohistochemistry (5-20 μg/mL). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects the 25 kDa Smac/DIABLO in samples from human, mouse, and rat origins.
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN223042) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-Smac/DIABLO polyclonal antibody in PBS ( pH 7.2) containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-DIABLO Antibody (ABIN223042)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Gesing, Masternak, Wang, Lewinski, Karbownik-Lewinska, Bartke: "Decreased expression level of apoptosis-related genes and/or proteins in skeletal muscles, but not in hearts, of growth hormone receptor knockout mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 236, Issue 2, pp. 156-68, 2011

Haudek, Taffet, Schneider, Mann: "TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2692-701, 2007

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN223042) back to top
Supplier Images
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (ABIN223042) Western blot analysis of Smac expression with human heart tissue lysate.