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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||159 Antibodies||61 Antibodies||41 Antibodies|
|Neutralization (Neut)||1 Antibodies||1 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||24 Antibodies||13 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||39 Antibodies||21 Antibodies||15 Antibodies|
|Immunohistochemistry (IHC)||56 Antibodies||38 Antibodies|
|Antigen||Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This DIABLO antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-DIABLO AntibodyTarget Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN223042) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412906|
|Specificity||The immunoaffinity purified antibody detects the 25 kDa Smac/DIABLO in samples from human, mouse, and rat origins.|
|Immunogen||Synthetic peptide surrounding amino acid 220 of mouse Smac/DIABLO|
Target Details DIABLOProduct Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN223042) Images back to top
|Alternative Name||Smac/DIABLO (DIABLO Antibody Abstract)|
Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Smac/DIABLO binds to IAPs (Inhibitor of Apoptosis Proteins) and neutralizes the inhibitory effect of IAPs. Analysis of the structural basis of Smac/DIABLO has revealed that the N-terminal amino acids are required for Smac/DIABLO to bind to IAPs and activation of caspases.
Synonyms: SMAC, SMAC3, DIABLO, DIABLO-S , FLJ10537, FLJ25049, 0610041G12Rik
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN223042) Images back to top
Western blotting (0.5-4 μg/mL) and Immunohistochemistry (5-20 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN223042) Images back to top
|Buffer||100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-Smac/DIABLO polyclonal antibody in PBS ( pH 7.2) containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-DIABLO Antibody (ABIN223042)Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
|Product cited in:||
Gesing, Masternak, Wang, Lewinski, Karbownik-Lewinska, Bartke: "Decreased expression level of apoptosis-related genes and/or proteins in skeletal muscles, but not in hearts, of growth hormone receptor knockout mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 236, Issue 2, pp. 156-68, 2011
Haudek, Taffet, Schneider, Mann: "TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways." in: The Journal of clinical investigation, Vol. 117, Issue 9, pp. 2692-701, 2007
ImagesProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN223042) back to top