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|Application / Reactivity||Human||Dog (Canine)||Hamster||Mouse (Murine)||Pig (Porcine)||Rat (Rattus)||Zebrafish (Danio rerio)||Chicken||Rabbit|
|Western Blotting (WB)||386 Antibodies||19 Antibodies||7 Antibodies||80 Antibodies||12 Antibodies||64 Antibodies||6 Antibodies||5 Antibodies||5 Antibodies|
|Light Microscopy (LM)||3 Antibodies|
|Immunoprecipitation (IP)||55 Antibodies||2 Antibodies||5 Antibodies||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||193 Antibodies||16 Antibodies||7 Antibodies|
|Antigen||Keratin 18 (KRT18) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus), Hamster, Rabbit, Chicken, Dog (Canine), Pig (Porcine), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This KRT18 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
|8 references available|
|Supplier||Log in to see|
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN335218) Images
|Specificity||Human, mouse, rat, hamster, rabbit, chicken, canine and swine.|
|Immunogen||RGE53 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal preparation of cells.|
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN335218) Images back to top
|Alternative Name||Cytokeratin 18 / Keratin K18 (KRT18 Antibody Abstract)|
|Background||Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN335218) Images back to top
|Application Notes||RGE53 reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. RGE53 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen sections and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and for flow cytometry. Optimal antibody dilution should be determined by titration.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN335218) Images back to top
References for anti-KRT18 Antibody (ABIN335218)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
|Product cited in:||
van Leenders, Dijkman, Hulsbergen-van de Kaa et al.: "Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 8, pp. 1251-8, 2000 (PubMed).
Ramaekers, Puts, Moesker et al.: "Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview." in: The Histochemical journal, Vol. 15, Issue 7, pp. 691-713, 1983 (PubMed).
Ramaekers, Huysmans, Moesker et al.: "Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 49, Issue 3, pp. 353-61, 1983 (PubMed).
Puts, Moesker, Kenemans et al.: "Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 4, Issue 4, pp. 300-13, 1986 (PubMed).
Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).
Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).
Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).
Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).
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