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anti-Human KRT18 antibody for Immunocytochemistry

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • cb83
  • CK18
  • CYK18
  • dapk1
  • DreK18
  • K18
  • Krt1-18
  • krt18
  • KRT18
  • krtt1c6
  • MGC64569
  • MGC75922
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • zgc:66015
Reactivity
Human
582
111
98
30
18
18
17
13
11
9
8
6
5
5
4
3
3
3
3
3
3
1
1
1
Host
Mouse
358
258
10
9
6
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
39
39
10
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
424
245
200
172
150
121
95
92
61
34
18
12
11
3
2
2
1
1
1
Supplier
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Catalog No. ABIN94285
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010100811 ABIN94282 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see DC-10 4
0.0010100811 ABIN94286 ICC Biotin Mouse IgG1 Log in to see DA-7 4
0.0010100811 ABIN959458 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see DE-K18
0.0010100811 ABIN2856142 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0010100811 ABIN959485 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see RCK106
0.0010100811 ABIN189287 ICC FACS IF IHC (p) IHC WB Rabbit IgG Log in to see E431-1
0.0010100811 ABIN1102771 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0010100811 ABIN959489 ICC IHC (fro) FACS WB Mouse IgG1 Log in to see RGE53
0.0010100811 ABIN256784 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010100811 ABIN462411 ICC IHC (p) IP ELISA WB Mouse IgG1 Log in to see DA-7
0.0010100811 ABIN462409 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see C-04
0.0010100811 ABIN1502291 ICC IHC (p) WB Mouse IgG1 Log in to see DC-10
0.0010100811 ABIN2169544 ICC WB Mouse IgG2a Log in to see 2B5-B6-F7
0.0010100811 ABIN335218 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RGE53 8
0.0010100811 ABIN462410 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see DC-10
0.0010100811 ABIN1723421 ICC IHC (p) IP ELISA WB Mouse IgG1 Log in to see DA-7
0.0010100811 ABIN335330 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RGE53 8
0.0010100811 ABIN449859 ICC IHC (fro) IF IHC ELISA WB Mouse IgG2b N-Term Log in to see SB38b
0.0010100811 ABIN227748 ICC FACS IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see
0.0010100811 ABIN1723420 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see DC-10

Top referenced anti-KRT18 antibody for Immunocytochemistry

Similar anti-KRT18 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 151 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 113 Antibodies
Immunochromatography (IC) 18 Antibodies
Immunocytochemistry (ICC) 86 Antibodies
Immunofluorescence (IF) 150 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 236 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 88 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 193 Antibodies
Immunoprecipitation (IP) 55 Antibodies

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human
(582), (111), (98), (30), (18), (18), (17), (13), (11), (9), (8), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Host Mouse
(358), (258), (10), (9), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(39), (39), (10), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(424), (245), (200), (172), (150), (121), (95), (92), (61), (34), (18), (12), (11), (3), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN94285) Images
Specificity The antibody DA-7 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
No Cross-Reactivity Pig (Porcine), Mouse (Murine), Rat (Rattus), Hamster, Cow (Bovine), Dog (Canine), Sheep (Ovine)
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Human breast carcinoma cell line PMC-42.
Clone DA-7
Isotype IgG1

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN94285) Images back to top
Antigen
Alternative Name KRT18 (KRT18 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratins 18 belongs to type I family (acidic cytokeratins).
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN94285) Images back to top
Application Notes Western Blotting: Recommended dilution: 1-2 µg/mL, overnight in 4 °C
Positive control: HeLa human cervix carcinoma cell lineA-431 human epidermoid carcinoma cell lineMCF-7 human breast adenocarcinoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions. SDS-PAGE (11 % separating gel).
Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Positive tissue: colon, skinImmunocytochemistry
Positive control: MCF-7 human breast adenocarcinoma cell lineHeLa human cervix carcinoma cell line
ELISA:
Positive control: MCF-7 human breast adenocarcinoma cell line
Application note: The antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 .

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN94285) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-KRT18 Antibody (ABIN94285)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Background publications

Taylor-Papadimitriou, Stampfer, Bartek, Lewis, Boshell, Lane, Leigh: "Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium." in: Journal of cell science, Vol. 94 ( Pt 3), pp. 403-13, 1990

Product cited in:

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, 1990

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN94285) back to top
Supplier Images
Western Blotting (WB) image for anti-KRT18 antibody (Keratin 18) (ABIN94285) Western Blotting analysis (reducing conditions) of whole cell lysate using anti-human...
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (ABIN94285) Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokerat...