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anti-Mammalian KRT18 antibody for ELISA

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • cb83
  • CK18
  • CYK18
  • dapk1
  • DreK18
  • K18
  • Krt1-18
  • krt18
  • KRT18
  • krtt1c6
  • MGC64569
  • MGC75922
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • zgc:66015
Reactivity
Mammalian
583
111
98
30
18
17
17
13
11
9
8
6
5
5
4
3
3
3
3
3
3
1
1
1
Host
Mouse
358
258
10
9
6
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
39
39
10
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
424
245
200
172
150
120
95
92
61
34
18
12
11
3
2
2
1
1
1
Supplier
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Catalog No. ABIN94279
$ 242.55
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012155632 ABIN1723419 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see C-04
0.0012155632 ABIN269268 ICC FACS IF IHC (p) IP IHC ELISA WB Biotin Mouse IgG1 Log in to see C-04
0.0012155632 ABIN268940 ICC FACS IF IHC (p) IP IHC ELISA WB Mouse IgG1 Log in to see C-04
0.0012155632 ABIN3200136 ELISA FACS ICC IHC (p) IP WB FITC Mouse IgG1 Log in to see C-04
0.0012155632 ABIN3200135 ELISA FACS ICC IHC (p) IP WB Biotin Mouse IgG1 Log in to see C-04
0.0012155632 ABIN3200134 ELISA FACS ICC IHC (p) IP WB Mouse IgG1 Log in to see C-04
0.0012155632 ABIN3037910 ICC FACS IF IP IHC ELISA WB Mouse IgG1 Log in to see
0.0012155632 ABIN3037912 ICC FACS IF IP IHC ELISA WB Biotin Mouse IgG1 Log in to see
0.0012155632 ABIN3037913 ICC FACS IF IP IHC ELISA WB FITC Mouse IgG1 Log in to see

Top referenced anti-KRT18 antibody for ELISA

  • Mammalian Monoclonal KRT18 Primary Antibody for ICC, FACS - ABIN94279 (6 Publications): Cap, Cerman, Nemecek, Marekova, Hana, Frysak: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. in Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 2003 (PubMed)

Similar anti-KRT18 Antibodies

Application / Reactivity Mammalian
ELISA 10 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 14 Antibodies

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Mammalian
(583), (111), (98), (30), (18), (17), (17), (13), (11), (9), (8), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Host Mouse
(358), (258), (10), (9), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(39), (39), (10), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(424), (245), (200), (172), (150), (120), (95), (92), (61), (34), (18), (12), (11), (3), (2), (2), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN94279) Images
Specificity The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Cytoskeleton preparation of epidermal carcinoma cell line A431.
Clone C-04
Isotype IgG1

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN94279) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratins 18 belongs to type I family (acidic cytokeratins).
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN94279) Images back to top
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Positive control: colon, kidney

Western Blotting: Recommended dilution: 1-2 µg/mL, overnight in 4 °C
Positive control: HeLa human cervix carcinoma cell line MCF-7 human breast adenocarcinoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions. SDS-PAGE (11 % separating gel).
Immunocytochemistry
ELISA:
Application note: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 .

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN94279) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-KRT18 Antibody (ABIN94279)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Background publications

Cap, Cerman, Nemecek, Marekova, Hana, Frysak: "The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 10, Issue 4, pp. 444-8, 2003

Taylor-Papadimitriou, Stampfer, Bartek, Lewis, Boshell, Lane, Leigh: "Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium." in: Journal of cell science, Vol. 94 ( Pt 3), pp. 403-13, 1990

Product cited in:

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, 1990

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN94279) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (ABIN94279) Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry stai...
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (ABIN94279) Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry stai...
Flow Cytometry (FACS) image for anti-KRT18 antibody (Keratin 18) (ABIN94279) Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of cytoke...