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anti-Rat (Rattus) KRT18 antibody for Immunocytochemistry

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • cb83
  • CK18
  • CYK18
  • dapk1
  • DreK18
  • K18
  • Krt1-18
  • krt18
  • KRT18
  • krtt1c6
  • MGC64569
  • MGC75922
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • zgc:66015
Reactivity
Human, Rat (Rattus)
899
166
143
57
55
32
29
25
20
19
19
18
13
12
7
7
7
5
4
3
3
3
1
1
1
Host
Rabbit
665
268
10
9
6
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
50
47
21
18
18
17
14
14
14
14
14
14
14
14
14
14
11
11
4
3
3
3
3
3
3
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
626
447
377
339
311
272
251
194
164
34
18
12
11
9
3
2
2
1
1
1
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Available images

Catalog No. ABIN189287
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965416 ABIN256784 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0009965416 ABIN335218 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RGE53 8
0.0009965416 ABIN335330 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RGE53 8
0.0009965416 ABIN1862826 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 72-380 Log in to see Polyclonal
0.0009965416 ABIN269268 ICC FACS IF IHC (p) IP IHC ELISA WB Biotin Mouse IgG1 Log in to see C-04
0.0009965416 ABIN268940 ICC FACS IF IHC (p) IP IHC ELISA WB Mouse IgG1 Log in to see C-04
0.0009965416 ABIN4302596 ELISA ICC IF IHC IHC (fro) IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0009965416 ABIN4302597 ELISA ICC IF IHC IHC (fro) IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0009965416 ABIN4302638 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 405 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302639 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 488 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302640 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 647 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302641 FACS ICC IF IHC IHC (fro) WB Alexa Fluor 700 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302626 FACS ICC IF IHC IHC (fro) WB DyLight 488 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302629 FACS ICC IF IHC IHC (fro) WB DyLight 405LS Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302631 FACS ICC IF IHC IHC (fro) WB DyLight 680 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302603 FACS ICC IF IHC IHC (fro) WB Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302607 FACS ICC IF IHC IHC (fro) WB DyLight 350 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302610 FACS ICC IF IHC IHC (fro) WB DyLight 405 Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302613 FACS ICC IF IHC IHC (fro) WB PE Mouse IgG1 Log in to see RGE53
0.0009965416 ABIN4302616 FACS ICC IF IHC IHC (fro) WB PerCP Mouse IgG1 Log in to see RGE53

Top referenced anti-KRT18 antibody for Immunocytochemistry

Similar anti-KRT18 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 26 Antibodies 250 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 10 Antibodies
Flow Cytometry (FACS) 53 Antibodies 369 Antibodies
Immunochromatography (IC) 1 Antibodies 18 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 56 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 52 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 81 Antibodies
Biochemical Assay (BCA) 2 Antibodies
FLISA 1 Antibodies

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human, Rat (Rattus)
(899), (166), (143), (57), (55), (32), (29), (25), (20), (19), (19), (18), (13), (12), (7), (7), (7), (5), (4), (3), (3), (3), (1), (1), (1)
Host Rabbit
(665), (268), (10), (9), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(50), (47), (21), (18), (18), (17), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (11), (11), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(626), (447), (377), (339), (311), (272), (251), (194), (164), (34), (18), (12), (11), (9), (3), (2), (2), (1), (1), (1)
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues of human Cytokeratin 18 was used as immunogen. Human Cytokeratin 18 (K18) was used as immunogen after isolation from cells pretreated with okadaic acid or pervanadate to promote Tyr hyperphosphorylation.
Clone E431-1
Isotype IgG

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteinsthat are expressed preferentially in glandular epithelia. Cytokeratins play a critical role indifferentiation and tissue specialization and function to maintain the overall structuralintegrity of epithelial cells (1-3). Together with actin microfilaments and microtubules,keratin filaments make up the cytoskeletons of vertebrate epithelial cells, formingalpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nmdiameter filaments (4). Alternate Names: anti-CK 18 antibody, anti-CK18 antibody, anti-CYK18 antibody, anti-Cytokeratin 18antibody, anti-Cytokeratin endo B antibody, anti-Cytokeratin18 antibody, anti-K18antibody, anti-Keratin 18 antibody, anti-Keratin D antibody, anti-Keratin type Icytoskeletal 18 antibody, anti-Keratin18 antibody, anti-Kerd antibody, anti-KRT18antibody.
Gene Symbol: KRT18
Gene ID 3875, 16668, 294853
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Flow Cytometry. In Western blot this antibody detects a band at approximately 45kDa. Use in Rat reported in customer review.
Recommended dilutions: Flow Cytometry 1:20, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:250, Western Blot 1:1000-10000
Protocol Protocol specific for Cytokeratin 18 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-KRT18 antibody (Keratin 18) (ABIN189287) Western blot analysis on A431 cell lysate using anti-Keratin 18 RabMAb (catalog #110-...
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (ABIN189287) Immunohistochemical analysis of paraffin-embedded colon carcinoma using anti-Keratin ...