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anti-Cow (Bovine) Lamin B1 antibody for Flow Cytometry

Recommended Lamin B1 Antibody (supplied by: Log in to see )

Antigen
Lamin B1 (LMNB1) Antibodies
  • ADLD
  • fc06g01
  • lamin-b
  • Lamin-L(I)
  • LMN
  • lmn
  • LMN2
  • lmn2
  • LMNB
  • lmnb
  • LMNB1
  • MGC52550
  • wu:fc06g01
Epitope
C-Term
18
10
9
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
127
54
49
10
7
6
5
5
4
3
2
1
1
1
Host
Mouse
83
34
19
Clonality
Monoclonal
Conjugate
This Lamin B1 antibody is un-conjugated
2
2
2
2
2
2
Application
Dot Blot (DB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
128
59
42
28
25
20
15
11
11
5
2
1
1
1
Supplier
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Catalog No. ABIN108542
$ 402.29
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010453054 ABIN360850 EIA FACS IHC (fro) IF WB Mouse IgG1 Log in to see 119D5-F1 8
0.0010453054 ABIN4330073 ELISA FACS ICC IF IHC IHC (fro) WB Mouse IgG1 Log in to see 119D5-F1 1
0.0010453054 ABIN335244 ICC FACS WB Mouse IgG1 Log in to see 119D5-F1 9
0.0010453054 ABIN335391 ICC FACS WB Mouse IgG1 Log in to see 119D5-F1 9
0.0010453054 ABIN2963329 ICC IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 119D5-F1

Top referenced anti-Lamin B1 antibody for Flow Cytometry

Similar anti-Lamin B1 Antibodies

Application / Reactivity Zebrafish (Danio rerio) Sheep (Ovine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 3 Antibodies 3 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 6 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 5 Antibodies
Immunofluorescence (IF) 3 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 6 Antibodies 6 Antibodies

General

Antigen Lamin B1 (LMNB1) Antibodies
Epitope C-Term
(18), (10), (9), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
(127), (54), (49), (10), (7), (6), (5), (5), (4), (3), (2), (1), (1), (1)
Host Mouse
(83), (34), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Lamin B1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Dot Blot (DB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(128), (59), (42), (28), (25), (20), (15), (11), (11), (5), (2), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-Lamin B1 Antibody

Target Details Lamin B1 Application Details Handling References for anti-Lamin B1 Antibody (ABIN108542) Images
Specificity Reacts with an epitope located C-terminal of residue 231 in lamin B1, does not cross react with lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. It has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested
No Cross-Reactivity Zebrafish
Purification Purified
Immunogen Purified rat liver lamins
Clone 119D5-F1
Isotype IgG1 kappa

Target Details Lamin B1

Product Details anti-Lamin B1 Antibody Application Details Handling References for anti-Lamin B1 Antibody (ABIN108542) Images back to top
Antigen
Alternative Name Lamin B1 (LMNB1 Antibody Abstract)
Background Synonyms:
Lamin B1 antibody,Lamin B2 antibody,LMN antibody,LMN B2 antibody,LMN2 antibody,LMNBantibody,LMNB1 antibody,LMNB2 antibody
Gene ID 540643, 474663, 4001
OMIM 150340
UniProt P70615
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Handling References for anti-Lamin B1 Antibody (ABIN108542) Images back to top
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try, Flow Cyt: 1/100 - 1/200. ICC: 1/20 - 1/50. WB: 1/100 - 1/1000. Predicted molecular weight: 67 kDa. (see Machiels et al reference and recommended protocol).
Restrictions For Research Use only

Handling

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details References for anti-Lamin B1 Antibody (ABIN108542) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Purified antibody in PBS containing 0.09 % sodium azide,
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-Lamin B1 Antibody (ABIN108542)

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details Handling Images back to top
Product cited in:

Ehrnhoefer, Skotte, Savill, Nguyen, Ladha, Cao, Dullaghan, Hayden: "A quantitative method for the specific assessment of caspase-6 activity in cell culture." in: PLoS ONE, Vol. 6, Issue 11, pp. e27680, 2011

Ciciarello, Roscioli, Di Fiore, Di Francesco, Sobrero, Bernard, Mangiacasale, Harel, Schininà, Lavia: "Nuclear reformation after mitosis requires downregulation of the Ran GTPase effector RanBP1 in mammalian cells." in: Chromosoma, Vol. 119, Issue 6, pp. 651-68, 2010

Kanninen, Heikkinen, Malm, Rolova, Kuhmonen, Leinonen, Ylä-Herttuala, Tanila, Levonen, Koistinaho, Koistinaho: "Intrahippocampal injection of a lentiviral vector expressing Nrf2 improves spatial learning in a mouse model of Alzheimer's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 38, pp. 16505-10, 2009

Hui, Francis, Guest, Costa, Gomes, Myatt, Brosens, Lam: "Doxorubicin activates FOXO3a to induce the expression of multidrug resistance gene ABCB1 (MDR1) in K562 leukemic cells." in: Molecular cancer therapeutics, Vol. 7, Issue 3, pp. 670-8, 2008

Hui, Gomes, Constantinidou, Costa, Karadedou, Fernandez de Mattos, Wymann, Brosens, Schulze, Lam: "The forkhead transcription factor FOXO3a increases phosphoinositide-3 kinase/Akt activity in drug-resistant leukemic cells through induction of PIK3CA expression." in: Molecular and cellular biology, Vol. 28, Issue 19, pp. 5886-98, 2008

Thompson, Winoto: "During negative selection, Nur77 family proteins translocate to mitochondria where they associate with Bcl-2 and expose its proapoptotic BH3 domain." in: The Journal of experimental medicine, Vol. 205, Issue 5, pp. 1029-36, 2008

Sharma, Brosh: "Human RECQ1 is a DNA damage responsive protein required for genotoxic stress resistance and suppression of sister chromatid exchanges." in: PLoS ONE, Vol. 2, Issue 12, pp. e1297, 2007

Ivorra, Kubicek, González, Sanz-González, Alvarez-Barrientos, OConnor, Burke, Andrés: "A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C." in: Genes & development, Vol. 20, Issue 3, pp. 307-20, 2006

Krohne: "Lamins." in: Methods in cell biology, Vol. 78, pp. 573-96, 2005

Johnson, Yu, Conrads, Kinoshita, Uo, Matthews, Lee, Smith, Veenstra, Morrison: "Proteome analysis of DNA damage-induced neuronal death using high throughput mass spectrometry." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26685-97, 2004

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997

Weaver, Carson, Walker, Chaly, Lach, Raymond, Brown, Sikorska: "Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes." in: Journal of cell science, Vol. 109 ( Pt 1), pp. 45-56, 1996

Images

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details Handling References for anti-Lamin B1 Antibody (ABIN108542) back to top
Supplier Images
 image for anti-Lamin B1 antibody (LMNB1) (C-Term) (ABIN108542) Immunostaining of lamin antibodies, (b) is Lamin B1 (clone 119D5-F1), with human epid...