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anti-Hamster Lamin B2 antibody for Western Blotting

Recommended Lamin B2 Antibody (supplied by: Log in to see )

Antigen
Lamin B2 (LMNB2) Antibodies
  • im:7142331
  • LAMB2
  • lamb2
  • lamin
  • lamin-b2
  • Lamin-L(II)
  • lmn2
  • LMN2
  • lmnb2
  • LMNB2
  • lmnb2-a
  • LOC100136040
  • RGD1563803
  • wu:fb94e05
  • wu:fb95e12
  • wu:fc15d06
  • wu:fc49h03
Epitope
C-Term
12
3
3
3
3
2
2
2
1
1
1
Reactivity
Mouse (Murine), Hamster, Human, Xenopus laevis, Zebrafish
62
30
24
6
4
4
3
2
2
1
1
1
Host
Mouse
50
17
Clonality
Monoclonal
Conjugate
This Lamin B2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
55
15
12
11
11
11
8
8
4
3
2
1
1
1
Supplier
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Catalog No. ABIN108634
$ 402.29
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728628 ABIN360851 FACS IHC (fro) WB Mouse IgG1 Log in to see LN43 8
0.0011728628 ABIN335245 FACS IHC WB Mouse IgG1 Log in to see LN43 8
0.0011728628 ABIN335392 FACS IHC WB Mouse IgG1 Log in to see LN43 8
0.0011728628 ABIN2963278 IHC (fro) FACS WB Mouse IgG1 Log in to see LN43

Top referenced anti-Lamin B2 antibody for Western Blotting

Similar anti-Lamin B2 Antibodies

Application / Reactivity Zebrafish Xenopus laevis Mouse (Murine)
Flow Cytometry (FACS) 1 Antibodies 5 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 2 Antibodies 1 Antibodies
Immunofluorescence (IF) 1 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 3 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 2 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 6 Antibodies
ELISA 4 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen Lamin B2 (LMNB2) Antibodies
Epitope C-Term
(12), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity Mouse (Murine), Hamster, Human, Xenopus laevis, Zebrafish
(62), (30), (24), (6), (4), (4), (3), (2), (2), (1), (1), (1)
Host Mouse
(50), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Lamin B2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(55), (15), (12), (11), (11), (11), (8), (8), (4), (3), (2), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-Lamin B2 Antibody

Target Details Lamin B2 Application Details Handling References for anti-Lamin B2 Antibody (ABIN108634) Images
Specificity Reacts with an epitope located in the C-terminal part of lamin B2.
Characteristics Myeloma fusion partners:
Mouse myeloma cells with spleen cells from a mouse
Detects a band of approximately 65-67 kDa.
Purification Purified
Immunogen Detergent insoluble fraction of potoroo cell line PtK1.
Clone LN43
Isotype IgG1

Target Details Lamin B2

Product Details anti-Lamin B2 Antibody Application Details Handling References for anti-Lamin B2 Antibody (ABIN108634) Images back to top
Antigen
Alternative Name Lamin B2 (LMNB2 Antibody Abstract)
Background Synonyms:
Lamin B1 antibody,Lamin B2 antibody,LMN antibody,LMN B2 antibody,LMN2 antibody,LMNBantibody,LMNB1 antibody,LMNB2 antibody
UniProt P21619
Research Area Signaling, Cytoskeleton, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Handling References for anti-Lamin B2 Antibody (ABIN108634) Images back to top
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try, ICC: 1/400. Flow Cyt: Use 1µg for 106 cells. IHC-Fr: 1/1 - 1/1. (when used with an avidin-biotinylated HRP complex as detection agent). WB: 1/100 - 1/1000.
Restrictions For Research Use only

Handling

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details References for anti-Lamin B2 Antibody (ABIN108634) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS containing 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C

References for anti-Lamin B2 Antibody (ABIN108634)

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling Images back to top
Product cited in:

Tsai, Lee, Lin: "Ribosome distribution in HeLa cells during the cell cycle." in: PLoS ONE, Vol. 7, Issue 3, pp. e32820, 2012

Shimi, Butin-Israeli, Adam, Hamanaka, Goldman, Lucas, Shumaker, Kosak, Chandel, Goldman: "The role of nuclear lamin B1 in cell proliferation and senescence." in: Genes & development, Vol. 25, Issue 24, pp. 2579-93, 2011

Taimen, Pfleghaar, Shimi, Möller, Ben-Harush, Erdos, Adam, Herrmann, Medalia, Collins, Goldman, Goldman: "A progeria mutation reveals functions for lamin A in nuclear assembly, architecture, and chromosome organization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20788-93, 2010

Ishimoto, Nakamura, Tachibana, Yamasaki, Ota, Hirano, Tanaka, Hamakubo, Sakai, Kodama, Doi: "Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22195-205, 2009

Mackay, Elgort, Ullman: "The nucleoporin Nup153 has separable roles in both early mitotic progression and the resolution of mitosis." in: Molecular biology of the cell, Vol. 20, Issue 6, pp. 1652-60, 2009

Hisatsune, Nakayama, Isomoto, Kurazono, Mukaida, Mukhopadhyay, Azuma, Yamaoka, Sap, Yamasaki, Yahiro, Moss, Hirayama et al.: "Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 5017-27, 2008

Koutsami, Tsantoulis, Kouloukoussa, Apostolopoulou, Pateras, Spartinou, Drougou, Evangelou, Kittas, Bartkova, Bartek, Gorgoulis: "Centrosome abnormalities are frequently observed in non-small-cell lung cancer and are associated with aneuploidy and cyclin E overexpression." in: The Journal of pathology, Vol. 209, Issue 4, pp. 512-21, 2006

Tsai, Wang, Heidinger, Shumaker, Adam, Goldman, Zheng: "A mitotic lamin B matrix induced by RanGTP required for spindle assembly." in: Science (New York, N.Y.), Vol. 311, Issue 5769, pp. 1887-93, 2006

Krohne: "Lamins." in: Methods in cell biology, Vol. 78, pp. 573-96, 2005

Background publications

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997

Images

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling References for anti-Lamin B2 Antibody (ABIN108634) back to top
Supplier Images
 image for anti-Lamin B2 antibody (LMNB2) (C-Term) (ABIN108634) Immunostaining of lamin antibodies (c) is Lamin B2 (clone LN43), with human epidermis.