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anti-Human Lamin B2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Lamin B2 Antibody (supplied by: Log in to see )

Antigen
Lamin B2 (LMNB2) Antibodies
  • im:7142331
  • LAMB2
  • lamb2
  • lamin
  • lamin-b2
  • Lamin-L(II)
  • lmn2
  • LMN2
  • lmnb2
  • LMNB2
  • lmnb2-a
  • LOC100136040
  • RGD1563803
  • wu:fb94e05
  • wu:fb95e12
  • wu:fc15d06
  • wu:fc49h03
Epitope
AA 221-500, Internal Region
13
3
3
3
2
2
2
1
1
1
1
Reactivity
Human
62
31
24
7
5
4
3
2
2
2
1
1
Host
Rabbit
49
18
Clonality
Polyclonal
Conjugate
This Lamin B2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
55
14
12
11
11
11
9
8
4
4
2
1
1
Supplier
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Catalog No. ABIN2800365
$ 431.20
Plus shipping costs $45.00

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Application / Reactivity Human
ELISA 9 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 52 Antibodies

General

Antigen Lamin B2 (LMNB2) Antibodies
Epitope AA 221-500, Internal Region
(13), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(62), (31), (24), (7), (5), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(49), (18)
Clonality
Polyclonal
Conjugate This Lamin B2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(55), (14), (12), (11), (11), (11), (9), (8), (4), (4), (2), (1), (1)
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Product Details anti-Lamin B2 Antibody

Target Details Lamin B2 Application Details Handling Images
Immunogen Recombinant fragment corresponding to a region within amino acids 221-500 of Lamin B2
Isotype IgG

Target Details Lamin B2

Product Details anti-Lamin B2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Lamin B2 (LMNB2 Antibody Abstract)
Background Synonyms: LAMB2 antibody, LMN2 antibody, MGC2721 antibody, LMNB2 antibody, lamin-B2 antibody, lamin B2 antibody
Molecular Weight 68 kDa
UniProt Q03252
Research Area Signaling, Cytoskeleton, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Handling Images back to top
Application Notes Tested IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.Lamin B2 antibody [N3C2], Internal detects LMNB2 protein at on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [N3C2], Internal (107-10877) dilution: 1:500.Lamin B2 antibody [N3C2], Internal immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [N3C2], Internal (107-10877) 7.5 % SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [N3C2], Internal (107-10877) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by Western blot analysis.A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract7.5 % SDS-PAGELamin B2 antibody [N3C2], Internal (107-10877) dilution: 1:500Sample (30 μg of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5 % SDS PAGE 107-10877 diluted at 1:1000
Comment

Lamin B2 antibody [N3C2], Internal, 1 mg/ml (100 μl)

Restrictions For Research Use only

Handling

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.