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|Application / Reactivity||Zebrafish||Xenopus laevis||Mouse (Murine)|
|Flow Cytometry (FACS)||1 Antibodies||5 Antibodies||5 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||2 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||2 Antibodies||7 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies||6 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Antigen||Lamin B2 (LMNB2) Antibodies|
|Reactivity||Mouse (Murine), Hamster, Human, Xenopus laevis, Zebrafish Alternatives|
|Conjugate||This Lamin B2 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|10 references available|
|Supplier||Log in to see|
Product Details anti-Lamin B2 AntibodyTarget Details Lamin B2 Application Details Handling References for anti-Lamin B2 Antibody (ABIN108634) Images
|Specificity||Reacts with an epitope located in the C-terminal part of lamin B2.|
Myeloma fusion partners:
Mouse myeloma cells with spleen cells from a mouse
Detects a band of approximately 65-67 kDa.
|Immunogen||Detergent insoluble fraction of potoroo cell line PtK1.|
Target Details Lamin B2Product Details anti-Lamin B2 Antibody Application Details Handling References for anti-Lamin B2 Antibody (ABIN108634) Images back to top
|Alternative Name||Lamin B2 (LMNB2 Antibody Abstract)|
Lamin B1 antibody,Lamin B2 antibody,LMN antibody,LMN B2 antibody,LMN2 antibody,LMNBantibody,LMNB1 antibody,LMNB2 antibody
|Research Area||Signaling, Cytoskeleton, Apoptosis/Necrosis|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Lamin B2 Antibody Target Details Lamin B2 Handling References for anti-Lamin B2 Antibody (ABIN108634) Images back to top
|Application Notes||Optimal antibody dilution should be determined by titration, however as a guideline try, ICC: 1/400. Flow Cyt: Use 1µg for 106 cells. IHC-Fr: 1/1 - 1/1. (when used with an avidin-biotinylated HRP complex as detection agent). WB: 1/100 - 1/1000.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details References for anti-Lamin B2 Antibody (ABIN108634) Images back to top
|Buffer||PBS containing 0.09 % sodium azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
References for anti-Lamin B2 Antibody (ABIN108634)Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling Images back to top
|Product cited in:||
Tsai, Lee, Lin: "Ribosome distribution in HeLa cells during the cell cycle." in: PLoS ONE, Vol. 7, Issue 3, pp. e32820, 2012 (PubMed).
Shimi, Butin-Israeli, Adam et al.: "The role of nuclear lamin B1 in cell proliferation and senescence." in: Genes & development, Vol. 25, Issue 24, pp. 2579-93, 2011 (PubMed).
Taimen, Pfleghaar, Shimi et al.: "A progeria mutation reveals functions for lamin A in nuclear assembly, architecture, and chromosome organization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20788-93, 2010 (PubMed).
Ishimoto, Nakamura, Tachibana et al.: "Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22195-205, 2009 (PubMed).
Mackay, Elgort, Ullman: "The nucleoporin Nup153 has separable roles in both early mitotic progression and the resolution of mitosis." in: Molecular biology of the cell, Vol. 20, Issue 6, pp. 1652-60, 2009 (PubMed).
Hisatsune, Nakayama, Isomoto et al.: "Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 5017-27, 2008 (PubMed).
Koutsami, Tsantoulis, Kouloukoussa et al.: "Centrosome abnormalities are frequently observed in non-small-cell lung cancer and are associated with aneuploidy and cyclin E overexpression." in: The Journal of pathology, Vol. 209, Issue 4, pp. 512-21, 2006 (PubMed).
Tsai, Wang, Heidinger et al.: "A mitotic lamin B matrix induced by RanGTP required for spindle assembly." in: Science (New York, N.Y.), Vol. 311, Issue 5769, pp. 1887-93, 2006 (PubMed).
Krohne: "Lamins." in: Methods in cell biology, Vol. 78, pp. 573-96, 2005 (PubMed).
Broers, Machiels, Kuijpers et al.: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997 (PubMed).
ImagesProduct Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling References for anti-Lamin B2 Antibody (ABIN108634) back to top