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anti-Zebrafish (Danio rerio) Lamin B2 antibody for Western Blotting

Recommended Lamin B2 Antibody (supplied by: Log in to see )

Antigen
Lamin B2 (LMNB2) Antibodies
  • im:7142331
  • LAMB2
  • lamb2
  • lamin
  • lamin-b2
  • Lamin-L(II)
  • lmn2
  • LMN2
  • lmnb2
  • LMNB2
  • lmnb2-a
  • LOC100136040
  • RGD1563803
  • wu:fb94e05
  • wu:fb95e12
  • wu:fc15d06
  • wu:fc49h03
Epitope
Middle Region
14
3
3
3
3
2
2
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
69
31
23
7
5
3
3
2
2
1
1
1
1
Host
Rabbit
53
21
Clonality
Polyclonal
Conjugate
This Lamin B2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
61
20
16
13
12
11
11
10
8
6
2
1
1
1
Supplier
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Catalog No. ABIN2782595
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728023 ABIN360851 FACS IHC (fro) WB Mouse IgG1 Log in to see LN43 8
0.0011728023 ABIN2963278 IHC (fro) FACS WB Mouse IgG1 Log in to see LN43

Top referenced anti-Lamin B2 antibody for Western Blotting

Similar anti-Lamin B2 Antibodies

Application / Reactivity Mouse (Murine) Human Xenopus laevis Chicken Cow (Bovine) Zebrafish (Danio rerio) Rat (Rattus)
Western Blotting (WB) 21 Antibodies 57 Antibodies 8 Antibodies 4 Antibodies 2 Antibodies 3 Antibodies 13 Antibodies
Immunoprecipitation (IP) 2 Antibodies 4 Antibodies 1 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies 6 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 20 Antibodies 7 Antibodies 3 Antibodies 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies 9 Antibodies

General

Antigen Lamin B2 (LMNB2) Antibodies
Epitope Middle Region
(14), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(69), (31), (23), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(53), (21)
Clonality
Polyclonal
Conjugate This Lamin B2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(61), (20), (16), (13), (12), (11), (11), (10), (8), (6), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Lamin B2 Antibody

Target Details Lamin B2 Application Details Handling References for anti-Lamin B2 Antibody (ABIN2782595) Images
Blocking Peptide Blocking peptide for this product available: ABIN979360
Sequence EVAMRTVKKS SVMRENENGE EEEEEAEFGE EDLFHQQGDP RTTSRGCYVM
Predicted Reactivity African clawed frog : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Chicken : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against LMNB2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human LMNB2

Target Details Lamin B2

Product Details anti-Lamin B2 Antibody Application Details Handling References for anti-Lamin B2 Antibody (ABIN2782595) Images back to top
Antigen
Alternative Name LMNB2 (LMNB2 Antibody Abstract)
Background The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. LMNB2 is one of the two B type proteins, B2. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene.
Molecular Weight 68 kDa
Gene ID 84823
NCBI Accession NM_032737, NP_116126
UniProt Q03252
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Handling References for anti-Lamin B2 Antibody (ABIN2782595) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 600 AA

Restrictions For Research Use only

Handling

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details References for anti-Lamin B2 Antibody (ABIN2782595) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Lamin B2 Antibody (ABIN2782595)

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling Images back to top
Background publications

Abdurashidova, Radulescu, Sandoval, Zahariev, Danailov, Demidovich, Santamaria, Biamonti, Riva, Falaschi: "Functional interactions of DNA topoisomerases with a human replication origin." in: The EMBO journal, Vol. 26, Issue 4, pp. 998-1009, 2007

Images

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling References for anti-Lamin B2 Antibody (ABIN2782595) back to top
Supplier Images
Western Blotting (WB) image for anti-Lamin B2 antibody (LMNB2) (Middle Region) (ABIN2782595) WB Suggested Anti-LMNB2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: