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anti-Rat (Rattus) NME1 antibody for Immunofluorescence

Recommended NME1 Antibody (supplied by: Log in to see )

Antigen
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
  • AL024257
  • AWD
  • CHH
  • GAAD
  • NB
  • NBR-A
  • NBR-B
  • NBS
  • NDKA
  • NDK I
  • NDPK-A
  • ndpk-b
  • NDPK-Z1
  • NDPKA
  • ndpkb
  • NDPK I
  • ndpkz1
  • NM23
  • NM23-B
  • NM23-C1
  • NM23-H1
  • nm23-h2
  • NM23-M1
  • NM23-Z1
  • NM23A
  • nm23b
  • nm23ndk
  • nm23ndk-a
  • nme1
  • NME1
  • NME1-1
  • NME1-NME2
  • nme2
  • nme2b1
  • puf
  • RMRPR
  • RRP2
  • T30A10.80
  • T30A10_80
Epitope
AA 44-152
27
21
14
13
13
12
11
7
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
269
37
36
12
6
6
3
2
2
1
1
Host
Mouse
140
129
2
2
2
Clonality
Monoclonal
Conjugate
This NME1 antibody is un-conjugated
12
11
8
7
6
6
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
214
116
75
56
34
11
10
10
8
7
2
2
2
1
Supplier
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Catalog No. ABIN967854
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3161046 ABIN2403524 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN2988568 ICC IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-NME1 antibody for Immunofluorescence

Similar anti-NME1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 2 Antibodies
ELISA 11 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 4 Antibodies
Immunofluorescence (IF) 3 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 4 Antibodies
Immunoprecipitation (IP) 2 Antibodies 2 Antibodies
Western Blotting (WB) 28 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
Epitope AA 44-152
(27), (21), (14), (13), (13), (12), (11), (7), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(269), (37), (36), (12), (6), (6), (3), (2), (2), (1), (1)
Host Mouse
(140), (129), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NME1 antibody is un-conjugated
(12), (11), (8), (7), (6), (6)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(214), (116), (75), (56), (34), (11), (10), (10), (8), (7), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-NME1 Antibody

Target Details NME1 Application Details Handling References for anti-NME1 Antibody (ABIN967854) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Nm23 aa. 44-152
Clone 56-Nm23
Isotype IgG2b

Target Details NME1

Product Details anti-NME1 Antibody Application Details Handling References for anti-NME1 Antibody (ABIN967854) Images back to top
Antigen
Alternative Name Nm23 (NME1 Antibody Abstract)
Background Nm23 is a potential metastasis suppressor protein whose expression is either reduced, altered (by mutation), or amplified in various types of metastatic carcinomas. Two human gene homologues, nm23-H1 and nm23-H2, encode 17 kDa proteins that are 90% identical. Nm23 proteins possess a nucleoside diphosphate kinase (NDPK) activity. This enzymatic activity catalyzes the synthesis of non-adenine-containing nucleoside triphosphates from nucleoside diphosphates via a phosphorylated enzyme intermediate. In addition, Nm23 inhibits differentiation, interacts with GTP-binding (GAP) proteins, autophosphorylates serine residues, and binds to DNA. The biochemical mechanisms by which Nm23 affects tumor metastatic potential have yet to be determined. In murine melanoma cell lines, serine 44 is the major site of autophosphorylation on Nm23-1. This acid-stable phosphorylation of Nm23 is inhibited in vitro by cAMP and in vivo by forskolin. These data indicate that this serine phosphorylation is regulated via some cAMP-dependent event in signal transduction. In addition, it has been shown that the Nm23-H2 protein is identical to the c-myc transcription factor PuF. This suggests that some of the cellular effects of Nm23 are mediated by its transcriptional regulatory function, while others are mediated by its NDPK activity.
Molecular Weight 17 kDa
Pathways Apoptosis

Application Details

Product Details anti-NME1 Antibody Target Details NME1 Handling References for anti-NME1 Antibody (ABIN967854) Images back to top
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-NME1 Antibody Target Details NME1 Application Details References for anti-NME1 Antibody (ABIN967854) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-NME1 Antibody (ABIN967854)

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling Images back to top
Product cited in:

MacDonald, De la Rosa, Benedict, Freije, Krutsch, Steeg: "A serine phosphorylation of Nm23, and not its nucleoside diphosphate kinase activity, correlates with suppression of tumor metastatic potential." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25780-9, 1994

Postel, Ferrone: "Nucleoside diphosphate kinase enzyme activity of NM23-H2/PuF is not required for its DNA binding and in vitro transcriptional functions." in: The Journal of biological chemistry, Vol. 269, Issue 12, pp. 8627-30, 1994

Rosengard, Krutzsch, Shearn, Biggs, Barker, Margulies, King, Liotta, Steeg: "Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development." in: Nature, Vol. 342, Issue 6246, pp. 177-80, 1989

Images

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling References for anti-NME1 Antibody (ABIN967854) back to top
Supplier Images
Western Blotting (WB) image for anti-NME1 antibody (Non-Metastatic Cells 1, Protein (NM23A) Expressed in) (AA 44-152) (ABIN967854) Western blot analysis of Nm23 on a HeLa cell lysate (Human cervical epitheloid carcin...
Immunofluorescence (IF) image for anti-NME1 antibody (Non-Metastatic Cells 1, Protein (NM23A) Expressed in) (AA 44-152) (ABIN967854) Immunofluorescence staining of human intestinal smooth muscle cells (HISM).