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|Antigen||Poly (ADP-Ribose) Polymerase 1 (PARP1) Antibodies|
|Epitope||AA 670-858 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PARP1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PARP1 AntibodyTarget Details PARP1 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Poly [ADP-ribose] polymerase 1(PARP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Poly [ADP-ribose] polymerase 1(PARP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: poly (ADP-ribose) polymerase 1
Protein Name: Poly [ADP-ribose] polymerase 1
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human PARP recombinant protein (Position: Q670-R858). Human PARP shares 94% and 95% amino acid (aa) sequence identity with mouse and rat PARP, respectively.|
Target Details PARP1Product Details anti-PARP1 Antibody Application Details Handling Images back to top
|Alternative Name||PARP1 (PARP1 Antibody Abstract)|
Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Synonyms: ADP ribosyltransferase (NAD+, poly (ADP ribose) polymerase) antibody|ADP ribosyltransferase diphtheria toxin like 1 antibody|ADP ribosyltransferase NAD(+) antibody|ADP ribosyltransferase NAD+ antibody|ADPRT 1 antibody|ADPRT antibody|ADPRT1 antibody|ARTD1 antibody|msPARP antibody|NAD(+) ADP ribosyltransferase 1 antibody|NAD(+) ADP-ribosyltransferase 1 antibody|pADPRT 1 antibody|pADPRT1 antibody|PARP 1 antibody|PARP antibody|PARP-1 antibody|PARP1 antibody|PARP1_HUMAN antibody|Poly (ADP ribose) polymerase 1 antibody|poly (ADP ribose) polymerase family, member 1 antibody|Poly [ADP-ribose]polymerase 1 antibody|Poly adenosine diphosphate ADP ribose polymerase antibody|Poly ADP ribose polymerase 1 antibody|Poly ADP ribose polymerase family member 1 antibody|Poly ADP ribose synthetase 1 antibody|Poly(ADP ribose) polymerase antibody|poly(ADP ribose) synthetase antibody|poly(ADP ribosyl)transferase antibody|Poly[ADP ribose] synthetase 1 antibody|Poly[ADP-ribose] synthase 1 antibody|PPOL antibody
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location|
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for PARP is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PARP1 Antibody Target Details PARP1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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