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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||25 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Immunoprecipitation (IP)||20 Antibodies|
|Western Blotting (WB)||120 Antibodies|
|Antigen||tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies|
|Epitope||AA 1-245, N-Term Alternatives|
|Conjugate||This YWHAQ antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-YWHAQ AntibodyTarget Details YWHAQ Application Details Handling References for anti-YWHAQ Antibody (ABIN336110) Images
|Specificity||The antibody recognizes Human 14-3-3 protein theta (tau). Other species not tested.|
|Characteristics||Synonyms: 14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1|
|Purification||Protein-G affinity chromatography|
|Immunogen||Recombinant human 14-3-3 tau (aa 1-245) purified from E. coli|
Target Details YWHAQProduct Details anti-YWHAQ Antibody Application Details Handling References for anti-YWHAQ Antibody (ABIN336110) Images back to top
|Alternative Name||14-3-3 Protein theta (YWHAQ Antibody Abstract)|
|Background||The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.Synonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ|
|Research Area||Transcription Factors|
Application DetailsProduct Details anti-YWHAQ Antibody Target Details YWHAQ Handling References for anti-YWHAQ Antibody (ABIN336110) Images back to top
ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-YWHAQ Antibody Target Details YWHAQ Application Details References for anti-YWHAQ Antibody (ABIN336110) Images back to top
|Buffer||PBS, pH 7.4, containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-YWHAQ Antibody (ABIN336110)Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling Images back to top
Liu, Elly, Yoshida, Bonnefoy-Berard, Altman: "Activation-modulated association of 14-3-3 proteins with Cbl in T cells." in: The Journal of biological chemistry, Vol. 271, Issue 24, pp. 14591-5, 1996
Xiao, Smerdon, Jones, Dodson, Soneji, Aitken, Gamblin: "Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways." in: Nature, Vol. 376, Issue 6536, pp. 188-91, 1995
ImagesProduct Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling References for anti-YWHAQ Antibody (ABIN336110) back to top