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anti-Guinea Pig C3 antibody for Immunofluorescence

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • AI255234
  • ARMD9
  • asp
  • ASP
  • c3
  • C3
  • C3-1
  • C3a
  • C3b
  • C3d
  • c3_D
  • CPAMD1
  • cpamd1
  • HSE-MSF
  • Plp
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
Reactivity
Guinea Pig
237
49
36
13
5
5
4
3
2
1
Host
Goat
102
100
83
13
8
7
Clonality
Polyclonal
Conjugate
This C3 antibody is conjugated to HRP
31
29
16
3
3
3
3
3
3
3
3
3
2
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
153
150
55
42
41
34
33
30
29
25
23
8
4
4
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN568554
$ 496.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010911712 ABIN568552 DB EIA ID IF IP WB Biotin Goat IgG Log in to see Polyclonal
0.0010911712 ABIN568553 DB EIA ID IF IP WB FITC Goat IgG Log in to see Polyclonal

Similar anti-C3 Antibodies

Application / Reactivity Guinea Pig
Dot Blot (DB) 3 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunodiffusion (ID) 4 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 4 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Guinea Pig
(237), (49), (36), (13), (5), (5), (4), (3), (2), (1)
Host Goat
(102), (100), (83), (13), (8), (7)
Clonality
Polyclonal
Conjugate This C3 antibody is conjugated to HRP
(31), (29), (16), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(153), (150), (55), (42), (41), (34), (33), (30), (29), (25), (23), (8), (4), (4), (3), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of guinea pig serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of guinea pig plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Characteristics Molar Ratio: Peroxidase/IgG ~1.2
Purification Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and DEAE-Chromatography
Immunogen C3c is isolated and purified from pooled normal guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3 is the most abundant complement protein in guinea pig serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response. The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain.Synonyms: CPAMD1, Complement component 3
Gene ID 24232
NCBI Accession NP_058690
UniProt P01026
Research Area Complement Factors
Pathways Complement System

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Reconstitution Restore by adding 1 mL of sterile distilled water.
Concentration 10,0 mg/mL
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.