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anti-Human C3 antibody for Immunohistochemistry (Frozen Sections)

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Human
279
70
58
13
6
5
5
3
3
1
Host
Mouse
140
112
91
25
19
8
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
32
32
20
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
196
191
70
69
51
50
49
44
43
35
33
24
11
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN111065
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN681913 IHC (fro) IF (p) IHC (p) Rabbit IgG AA 1611-1631 Log in to see Polyclonal 2
1 ABIN317012 EIA FACS IHC (fro) WB Mouse IgG2b Log in to see 013III-1-16 3
1 ABIN112153 EIA IHC (fro) WB Mouse IgG1 Log in to see H206 1
1 ABIN181107 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (1003) 2
1 ABIN181106 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 10-02A 1
1 ABIN181129 EIA FACS IHC (fro) Mouse IgG1 Log in to see 053A-514-3-1-4 2
1 ABIN111064 EIA IHC (fro) WB Mouse IgG2b Log in to see H13 3
1 ABIN784157 EIA IHC (fro) IHC (p) Sheep IgG Log in to see Polyclonal
1 ABIN1106776 EIA IHC (fro) IHC (p) Mouse IgG2a Log in to see bH6 2
1 ABIN568570 DB EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN568572 DB EIA ID IHC (fro) IF IP WB HRP Goat IgG Log in to see Polyclonal
1 ABIN568571 EIA ID IHC (fro) IF IP FITC Goat IgG Log in to see Polyclonal
1 ABIN568574 EIA ID IHC (fro) IF IP TRITC Goat IgG Log in to see Polyclonal
1 ABIN121477 IHC (fro) FITC Sheep Log in to see Polyclonal
1 ABIN345336 ICC IHC (fro) WB Mouse IgG1 Log in to see H11
1 ABIN215710 IHC (fro) WB Mouse IgG2b Log in to see H13
1 ABIN601079 IHC (fro) FACS ELISA WB Mouse IgG2b Log in to see 013III-1-16
1 ABIN601080 IHC (fro) WB Mouse IgG1 Log in to see
1 ABIN601081 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see
1 ABIN609442 IHC (fro) IHC (p) ELISA Mouse IgG2a Log in to see bH6

Top referenced anti-C3 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-C3 Antibodies

Application / Reactivity Human
Dot Blot (DB) 2 Antibodies
ELISA 139 Antibodies
Enzyme Immunoassay (EIA) 29 Antibodies
Flow Cytometry (FACS) 31 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 5 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunodiffusion (ID) 12 Antibodies
Immunoelectrophoresis (IEP) 1 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 8 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 36 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 19 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Human
(279), (70), (58), (13), (6), (5), (5), (3), (3), (1)
Host Mouse
(140), (112), (91), (25), (19), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(32), (32), (20), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(196), (191), (70), (69), (51), (50), (49), (44), (43), (35), (33), (24), (11), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 Antibody (ABIN111065) Images
Specificity Detects C3b deposits in tissue, on cells, on microorganisms and in immune complexes. Does not react with C3a or C3d. Inhibits hemolytic function of C3. It should be used with positive control anti C3b-α (clone H206, cat.no. BM5029), and negative control anti C3a (clone H13, cat.no. BM2131). Activated, deposited C3b bears 3b-α and -ß, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with clone H13.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein A affinity chromatography
Immunogen Human complement component C3
Clone H11
Isotype IgG1

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 Antibody (ABIN111065) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
Gene ID 718
NCBI Accession NP_000055
UniProt P01024
Research Area Complement Factors
Pathways Complement System

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 Antibody (ABIN111065) Images back to top
Application Notes Western Blot. Immunohistochemistry on frozen tissue (Dilution 1: 10, Incubation time: 1h at RT). Successful use on paraffin embedded tissue was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 Antibody (ABIN111065) Images back to top
Reconstitution Restore with 1 mL dist. water.
Buffer PBS, pH 7.4, containing 0.09 % sodium azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-C3 Antibody (ABIN111065)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Background publications

Burger, Bader, Kirschfink, Rother, Schrod, Wörner, Zilow: "Functional analysis and quantification of the complement C3 derived anaphylatoxin C3a with a monoclonal antibody." in: Clinical and experimental immunology, Vol. 68, Issue 3, pp. 703-11, 1987

Sugita, Majdic, Stockinger, Holter, Burger, Knapp: "Induction of human complement activation without cytolysis by mouse monoclonal antibodies to human leukocyte antigens." in: Transplantation, Vol. 43, Issue 4, pp. 570-4, 1987