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|Application / Reactivity||Human|
|Dot Blot (DB)||2 Antibodies|
|Enzyme Immunoassay (EIA)||29 Antibodies|
|Flow Cytometry (FACS)||26 Antibodies|
|Immunoassay (IA)||4 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunodiffusion (ID)||12 Antibodies|
|Immunoelectrophoresis (IEP)||1 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||36 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies|
|Immunoprecipitation (IP)||16 Antibodies|
|Immunoturbidimetry (IT)||1 Antibodies|
|Labeling (Lbl)||1 Antibodies|
|Radial Immunodiffusion (RID)||1 Antibodies|
|Radioimmunoassay (RIA)||4 Antibodies|
|Antigen||Complement Component 3 (C3) Antibodies|
|Conjugate||This C3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP)
|5 references available|
|Supplier||Log in to see|
Product Details anti-C3 AntibodyTarget Details C3 Application Details Handling References for anti-C3 Antibody (ABIN1107627) Images
|Specificity||The monocolonal antibody 9 reacts with a neoantigen on iC3, iC3b, C3dg and C3g. It recognizes iC3b, C3dg and C3g in plasma. The antibody does not recognize C3 or C3b.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human|
Target Details C3Product Details anti-C3 Antibody Application Details Handling References for anti-C3 Antibody (ABIN1107627) Images back to top
|Alternative Name||Complement C3 (C3 Antibody Abstract)|
|Background||C3g itself however is a small fragment probably not formed in vivo. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/mL. An inherited deficiency of C3 predisposes the person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by C3-convertases results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b and C3f. iC3b is further processed into C3c and C3dg. C3dg can be cleaved into C3d and C3g though this does not occur in plasma.Synonyms: Complement C3|
|Research Area||Complement Factors|
Application DetailsProduct Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 Antibody (ABIN1107627) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 Antibody (ABIN1107627) Images back to top
|Buffer||PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store at 2 - 8 °C.|
References for anti-C3 Antibody (ABIN1107627)Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Lachmann, Voak, Oldroyd et al.: "Use of monoclonal anti-C3 antibodies to characterise the fragments of C3 that are found on erythrocytes." in: Vox sanguinis, Vol. 45, Issue 5, pp. 367-72, 1983 (PubMed).
Chaplin, Monroe, Lachmann: "Further studies of the C3g component of the alpha 2D fragment of human C3." in: Clinical and experimental immunology, Vol. 51, Issue 3, pp. 639-46, 1983 (PubMed).
Lachmann, Pangburn, Oldroyd: "Breakdown of C3 after complement activation. Identification of a new fragment C3g, using monoclonal antibodies." in: The Journal of experimental medicine, Vol. 156, Issue 1, pp. 205-16, 1982 (PubMed).
Lachmann, Oldroyd, Milstein et al.: "Three rat monoclonal antibodies to human C3." in: Immunology, Vol. 41, Issue 3, pp. 503-15, 1981 (PubMed).
Mollnes, Lachmann: "Activation of the third component of complement (C3) detected by a monoclonal anti-C3'g' neoantigen antibody in a one-step enzyme immunoassay." in: Journal of immunological methods, Vol. 101, Issue 2, pp. 201-7, 1987 (PubMed).