You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) C3 antibody for Immunoassay

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • AI255234
  • ARMD9
  • asp
  • ASP
  • c3
  • C3
  • C3-1
  • C3a
  • C3b
  • C3d
  • c3_D
  • CPAMD1
  • cpamd1
  • HSE-MSF
  • Plp
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
Reactivity
Mouse (Murine)
237
49
36
13
5
5
5
3
2
1
Host
Rat
102
100
84
13
8
7
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
31
29
17
3
3
3
3
3
3
3
3
3
2
1
Application
Immunoassay (IA), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
153
151
55
43
42
35
33
30
29
25
24
8
5
4
3
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN152429
Contact our Customer Service for availability and price in your country.

Similar anti-C3 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Western Blotting (WB) 26 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Mouse (Murine)
(237), (49), (36), (13), (5), (5), (5), (3), (2), (1)
Host Rat
(102), (100), (84), (13), (8), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(31), (29), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Immunoassay (IA), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(153), (151), (55), (43), (42), (35), (33), (30), (29), (25), (24), (8), (5), (4), (3), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity Antibody recognizes mouse complement component C3 and its activation products, C3b, iC3b, C3d and C3dg
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Immunogen C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)
Clone 11H9
Isotype IgG2a

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background In eukaryotic cells, DNA is associated with histones and other proteins to formchromatin. The cell division cycle constitutes a series of processes that have evolved tocreate two genetically identical daughter cells from a mother cell. One of theseprocesses is the conversion of relatively amorphous, extended interphase chromatininto condensed, highly ordered mitotic chromosomes. Proper mitotic chromosomecondensation is essential for the correct segregation of sister chromatids into twodaughter cells. The basic unit of chromatin is the nucleosome core particle, whichconsists of 140 bp DNA wrapped around an octameric core containing two each of thefour conserved core histones: H2A, H2B, H3 and H4. A fifth histone, the linker histoneH1, interacts with DNA of variable length, linking adjacent nucleosome cores, andfurther compacting thechromatin. Chromatin changes are initiated during G2 phase of the cell cycle, inpreparation for cell division. The moststriking morphological change is chromatin condensation, which becomes apparentduring prophase and is maximal during the subsequent stages of mitosis. Histone H1and the N-terminal tail of H3 have key roles in the folding and inter-association of thechromatin fiber. Two currently known phosphorylation sites are present in theN-terminus of H3, serine-10 and serine-28.1,2Mitogenic stimulation, oncogenic transformation, or induction of oncogenic rasexpression are accompanied with increase in serine-10 phosphorylation of the H3N-terminal domain. Indeed, it has been shown that phosphorylated H3 is associatedwith c-fos andc-myc genes in stimulated cells. Phosphorylation of H3, at both serine-10 and -28,coincides with the induction of mitotic chromosome condensation. H3 phosphorylationmay contribute to proto-oncogene induction by modulating chromatin structure andreleasing blocks in elongation. In contrast to H1 hyperphosphorylation, site-specificphosphorylation of core histone H3 at serine-10 and -28 appears to occur exclusivelyduring mitosis in mammalian cells. H3 dephosphorylation occurs quite rapidly aftermitosis and serine-10/28 remain unphosphorylated throughout the remainder ofinterphase. PP1 has been identified as the H3 phosphatase. Monoclonal antibodiesreacting specifically with phosphorylated histone H3, are useful tools to study molecularmechanisms associated with the G2 to M transition and chromatin condensation, and forthe analysis of protein kinase(s) and phosphatase(s) involved in H3 phosphorylation ordephosphorylation. They may also be used in multiparameter analysis to relate H3phosphorylation in ndividual cells to the cell. Alternate Names: anti-Acylation stimulating protein cleavage product antibody, anti-ASP antibody,anti-Complement C3 antibody, anti-Complement C3 precursor antibody,anti-Complement component 3 antibody, anti-CPAMD 1 antibody, anti-CPAMD1antibody.
Gene Symbol: C3
Gene ID 718
Research Area Complement Factors
Pathways Complement System

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes For IHC-F: Tissue sections were fixed in acetone. Antigen retrieval was performed using 50% formic acid for 5 min or microwaved (700 W) in antigen unmasking solution. For IHC-P: free-floating brain tissue pretreated with 50% formic acid for 5 min. or microwaved (700W) in antigen unmasking solution for 2-5min. Blocking endogenous persoxidase by treatment with 3% H2O2 in pBS 20 min at 25 degrees C. Blocking 2hrs, 2% BSA in PBS. For IF: Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Recommended dilutions: Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunoprecipitation 1:10-1:500, Immunoassay 0.5 µg/well in PBS, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Concentration 0.1 mg/mL
Buffer PBS with 0.1 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-C3 antibody (Complement Component 3) (ABIN152429) anti-Complement Component 3 (C3) antibody