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anti-Mouse (Murine) C3 antibody for Immunoassay

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Mouse (Murine)
265
72
61
15
7
6
5
4
4
1
1
1
Host
Rat
134
111
91
24
14
8
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
31
29
18
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunoassay (IA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
193
182
69
64
59
51
45
42
41
39
35
23
10
5
4
3
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN152429
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2191788 IA FACS IF IP WB Rat IgG2a Log in to see 11H9 1
1 ABIN4299771 CyTOF FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see 11H9
1 ABIN4299787 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Rat IgG2a Log in to see 11H9

Top referenced anti-C3 antibody for Immunoassay

Similar anti-C3 Antibodies

Application / Reactivity Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 19 Antibodies
Immunoassay (IA) 4 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Immunosubtraction (ISub) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 48 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Mouse (Murine)
(265), (72), (61), (15), (7), (6), (5), (4), (4), (1), (1), (1)
Host Rat
(134), (111), (91), (24), (14), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(31), (29), (18), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunoassay (IA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(193), (182), (69), (64), (59), (51), (45), (42), (41), (39), (35), (23), (10), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 Antibody (ABIN152429) Images
Specificity Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg
Purification Protein G purified
Immunogen C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)
Clone 11H9
Isotype IgG2a

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 Antibody (ABIN152429) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background Gene Symbol: C3
Gene ID 718
UniProt P01027
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 Antibody (ABIN152429) Images back to top
Application Notes Western Blot 1:100-1:2000, Flow Cytometry 1-2 μg/mL, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Immunoassay 0.5 μg/well in PBS, CyTOF-readyFor IHC-F: Tissue sections were fixed in acetone. Antigen retrieval was performed using 50 % formic acid for 5 min or microwaved (700 W) in antigen unmasking solution. For IHC-P: free-floating brain tissue pretreated with 50 % formic acid for 5 min. or microwaved (700W) in antigen unmasking solution for 2-5 min. Blocking endogenous persoxidase by treatment with 3 % H2O2 in pBS 20 min at 25 degrees C. Blocking 2hrs, 2 % BSA in PBS. For IF: Fixation in 4 % paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3 % hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 Antibody (ABIN152429) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-C3 Antibody (ABIN152429)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Product cited in:

Maehara, Arai, Mori, Iwamura, Kurokawa, Kai, Kusunoki, Taniguchi, Ikeda, Ohara, Yamamura, Miyazaki: "Circulating AIM prevents hepatocellular carcinoma through complement activation." in: Cell reports, Vol. 9, Issue 1, pp. 61-74, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling References for anti-C3 Antibody (ABIN152429) back to top
Supplier Images
Immunofluorescence (IF) image for anti-C3 antibody (Complement Component 3) (ABIN152429) anti-Complement Component 3 (C3) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-C3 antibody (Complement Component 3) (ABIN152429) Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [ABIN152429] - IHC-P det...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-C3 antibody (Complement Component 3) (ABIN152429) Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [ABIN152429] - IHC-P det...
 image for anti-C3 antibody (Complement Component 3) (ABIN152429) Flow (Intracellular): Complement C3 Antibody (11H9) [ABIN152429] - An intracellular s...