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anti-Mouse (Murine) C3 antibody for Immunohistochemistry

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • AI255234
  • ARMD9
  • asp
  • ASP
  • c3
  • C3
  • C3-1
  • C3a
  • C3b
  • C3d
  • c3_D
  • CPAMD1
  • cpamd1
  • HSE-MSF
  • Plp
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
Reactivity
Mouse (Murine)
243
65
55
18
13
5
5
5
3
2
1
Host
Rat
141
102
86
24
13
7
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
34
31
20
6
6
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunoassay (IA), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
193
188
71
67
59
45
44
43
40
35
33
24
20
5
4
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN152429
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011307208 ABIN958049 IHC WB Goat IgG Log in to see Polyclonal
0.0011307208 ABIN1173824 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011307208 ABIN4299771 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see 11H9
0.0011307208 ABIN2236802 FACS IF IP IHC ELISA WB Rat IgG2a Log in to see 6A525
0.0011307208 ABIN4299773 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299774 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299775 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Allophycocyanin Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299776 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Biotin Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299777 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 350 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299778 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299779 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405LS Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299780 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 488 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299781 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 550 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299782 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 650 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299783 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 680 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299784 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 755 Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299785 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB HRP Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299786 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB PE Rat IgG2a Log in to see 11H9
0.0011307208 ABIN4299787 FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Rat IgG2a Log in to see 11H9
0.0011307208 ABIN2236803 IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-C3 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Immunoassay (IA) 17 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Western Blotting (WB) 42 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Mouse (Murine)
(243), (65), (55), (18), (13), (5), (5), (5), (3), (2), (1)
Host Rat
(141), (102), (86), (24), (13), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(34), (31), (20), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunoassay (IA), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(193), (188), (71), (67), (59), (45), (44), (43), (40), (35), (33), (24), (20), (5), (4), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity Antibody recognizes mouse complement component C3 and its activation products, C3b, iC3b, C3d and C3dg
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Immunogen C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)
Clone 11H9
Isotype IgG2a

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background In eukaryotic cells, DNA is associated with histones and other proteins to formchromatin. The cell division cycle constitutes a series of processes that have evolved tocreate two genetically identical daughter cells from a mother cell. One of theseprocesses is the conversion of relatively amorphous, extended interphase chromatininto condensed, highly ordered mitotic chromosomes. Proper mitotic chromosomecondensation is essential for the correct segregation of sister chromatids into twodaughter cells. The basic unit of chromatin is the nucleosome core particle, whichconsists of 140 bp DNA wrapped around an octameric core containing two each of thefour conserved core histones: H2A, H2B, H3 and H4. A fifth histone, the linker histoneH1, interacts with DNA of variable length, linking adjacent nucleosome cores, andfurther compacting thechromatin. Chromatin changes are initiated during G2 phase of the cell cycle, inpreparation for cell division. The moststriking morphological change is chromatin condensation, which becomes apparentduring prophase and is maximal during the subsequent stages of mitosis. Histone H1and the N-terminal tail of H3 have key roles in the folding and inter-association of thechromatin fiber. Two currently known phosphorylation sites are present in theN-terminus of H3, serine-10 and serine-28.1,2Mitogenic stimulation, oncogenic transformation, or induction of oncogenic rasexpression are accompanied with increase in serine-10 phosphorylation of the H3N-terminal domain. Indeed, it has been shown that phosphorylated H3 is associatedwith c-fos andc-myc genes in stimulated cells. Phosphorylation of H3, at both serine-10 and -28,coincides with the induction of mitotic chromosome condensation. H3 phosphorylationmay contribute to proto-oncogene induction by modulating chromatin structure andreleasing blocks in elongation. In contrast to H1 hyperphosphorylation, site-specificphosphorylation of core histone H3 at serine-10 and -28 appears to occur exclusivelyduring mitosis in mammalian cells. H3 dephosphorylation occurs quite rapidly aftermitosis and serine-10/28 remain unphosphorylated throughout the remainder ofinterphase. PP1 has been identified as the H3 phosphatase. Monoclonal antibodiesreacting specifically with phosphorylated histone H3, are useful tools to study molecularmechanisms associated with the G2 to M transition and chromatin condensation, and forthe analysis of protein kinase(s) and phosphatase(s) involved in H3 phosphorylation ordephosphorylation. They may also be used in multiparameter analysis to relate H3phosphorylation in ndividual cells to the cell. Alternate Names: anti-Acylation stimulating protein cleavage product antibody, anti-ASP antibody,anti-Complement C3 antibody, anti-Complement C3 precursor antibody,anti-Complement component 3 antibody, anti-CPAMD 1 antibody, anti-CPAMD1antibody.
Gene Symbol: C3
Gene ID 718
Research Area Complement Factors
Pathways Complement System

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes For IHC-F: Tissue sections were fixed in acetone. Antigen retrieval was performed using 50% formic acid for 5 min or microwaved (700 W) in antigen unmasking solution. For IHC-P: free-floating brain tissue pretreated with 50% formic acid for 5 min. or microwaved (700W) in antigen unmasking solution for 2-5min. Blocking endogenous persoxidase by treatment with 3% H2O2 in pBS 20 min at 25 degrees C. Blocking 2hrs, 2% BSA in PBS. For IF: Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Recommended dilutions: Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunoprecipitation 1:10-1:500, Immunoassay 0.5 µg/well in PBS, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Concentration 0.1 mg/mL
Buffer PBS with 0.1 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-C3 antibody (Complement Component 3) (ABIN152429) anti-Complement Component 3 (C3) antibody