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anti-Non-Human Primate CD55 antibody for Flow Cytometry

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • AI323748
  • Cd55b
  • CR
  • CROM
  • Daf
  • DAF
  • Daf-GPI
  • Daf-TM
  • Daf1
  • GPI-DAF
  • TC
  • TM-DAF
Epitope
Domain SCR4
16
15
8
8
7
6
6
5
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Non-Human Primate
413
42
19
9
7
3
3
1
Host
Mouse
341
91
13
8
7
3
2
Clonality
Monoclonal
Conjugate
This CD55 antibody is conjugated to Biotin
54
51
29
10
10
8
8
8
8
7
6
6
6
6
6
6
6
5
5
4
3
3
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS)
353
178
79
64
58
54
49
41
12
5
4
3
3
2
1
1
1
1
Supplier
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Catalog No. ABIN94184
$ 288.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30402437 ABIN1027693 FACS IHC (p) IP PE Mouse IgM Domain SCR4 Log in to see MEM-118 6
0.30402437 ABIN94185 FACS FITC Mouse IgM Domain SCR4 Log in to see MEM-118 6
0.30402437 ABIN94183 FACS IHC (p) IP Mouse IgM Domain SCR4 Log in to see MEM-118 6

Top referenced anti-CD55 antibody for Flow Cytometry

Similar anti-CD55 Antibodies

Application / Reactivity Human Non-Human Primate
Western Blotting (WB) 157 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 5 Antibodies
Immunoprecipitation (IP) 51 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 52 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunofluorescence (IF) 54 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Functional Studies (Func) 3 Antibodies
Flow Cytometry (FACS) 322 Antibodies

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Epitope Domain SCR4
(16), (15), (8), (8), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Non-Human Primate
(413), (42), (19), (9), (7), (3), (3), (1)
Host Mouse
(341), (91), (13), (8), (7), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is conjugated to Biotin
(54), (51), (29), (10), (10), (8), (8), (8), (8), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS)
(353), (178), (79), (64), (58), (54), (49), (41), (12), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN94184) Images
Specificity The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells, it is weakly present on NK cells.HLDA V, WS Code AS S016
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Purification Purified from ascites by protein-A affinity chromatography.
Immunogen HPB-ALL human T cell line
Clone MEM-118
Isotype IgM

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling References for anti-CD55 Antibody (ABIN94184) Images back to top
Antigen
Alternative Name CD55 (CD55 Antibody Abstract)
Background CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling References for anti-CD55 Antibody (ABIN94184) Images back to top
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 1: 500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details References for anti-CD55 Antibody (ABIN94184) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD55 Antibody (ABIN94184)

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
Background publications

VanLandingham, Cekic, Cutler, Hoffman, Stein: "Neurosteroids reduce inflammation after TBI through CD55 induction." in: Neuroscience letters, Vol. 425, Issue 2, pp. 94-8, 2007

Abbott, Spendlove, Roversi, Fitzgibbon, Knott, Teriete, McDonnell, Handford, Lea: "Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 22023-32, 2007

Miwa, Maldonado, Zhou, Yamada, Gilkeson, Eisenberg, Song: "Decay-accelerating factor ameliorates systemic autoimmune disease in MRL/lpr mice via both complement-dependent and -independent mechanisms." in: The American journal of pathology, Vol. 170, Issue 4, pp. 1258-66, 2007

Mikesch, Buerger, Simon, Brandt: "Decay-accelerating factor (CD55): a versatile acting molecule in human malignancies." in: Biochimica et biophysica acta, Vol. 1766, Issue 1, pp. 42-52, 2006

Miwa, Maldonado, Zhou, Sun, Luo, Cai, Werth, Madaio, Eisenberg, Song: "Deletion of decay-accelerating factor (CD55) exacerbates autoimmune disease development in MRL/lpr mice." in: The American journal of pathology, Vol. 161, Issue 3, pp. 1077-86, 2002

Product cited in:

Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999

Images

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN94184) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD55 antibody (Complement Decay-Accelerating Factor) (Domain SCR4) (Biotin) (ABIN94184) Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD55 a...