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anti-Human CFP antibody for Radial Immunodiffusion

Recommended CFP Antibody (supplied by: Log in to see )

Antigen
Complement Factor P (CFP) Antibodies
  • BCFG
  • BFD
  • PFC
  • Pfc
  • PFD
  • Properdin
  • PROPERDIN
Reactivity
Human
106
30
21
18
2
1
1
1
Host
Goat
76
54
13
Clonality
Polyclonal
Conjugate
This CFP antibody is un-conjugated
13
13
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
82
80
16
12
12
11
9
9
7
5
4
4
2
2
2
1
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Catalog No. ABIN457968
$ 217.63
Plus shipping costs $45.00

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Application / Reactivity Human
ELISA 56 Antibodies
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Functional Studies (Func) 6 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunoelectrophoresis (IEP) 1 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Radial Immunodiffusion (RID) 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen Complement Factor P (CFP) Antibodies
Reactivity Human
(106), (30), (21), (18), (2), (1), (1), (1)
Host Goat
(76), (54), (13)
Clonality
Polyclonal
Conjugate This CFP antibody is un-conjugated
(13), (13), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
(82), (80), (16), (12), (12), (11), (9), (9), (7), (5), (4), (4), (2), (2), (2), (1)
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Product Details anti-CFP Antibody

Target Details CFP Application Details Handling Images
Specificity The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
Characteristics Precipitating polyclonal goat antiserum to human properdin
Purification Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Immunogen Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml. The antigen has been isolated form pooled human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details CFP

Product Details anti-CFP Antibody Application Details Handling Images back to top
Antigen
Alternative Name Properdin (CFP Antibody Abstract)
Background The defined antibody specificity is restricted to properdin as tested at the level of sensitivity of immunoprecipitation techniques. A single precipitin line is obtained in immuno-electrophoresis, bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified properdin. No reaction is obtained with any other plasma protein
Pathways Complement System

Application Details

Product Details anti-CFP Antibody Target Details CFP Handling Images back to top
Application Notes In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
Restrictions For Research Use only

Handling

Product Details anti-CFP Antibody Target Details CFP Application Details Images back to top
Format Lyophilized
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:8 when tested against pooled normal human plasma in agar-block immunodiffusion titrati
Buffer Delipidated, heat inactivated, lyophilized, stable whole antiserum
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.