In Immunoelectrophoresis against Human EDTA plasma a single precipitin line is obtained in the beta-2 region. The antiserum does not react with any other protein component of human serum or plasma. Cross-Reactivity: The antiserum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in Double Radial Immunodiffusion a weak reaction with Mouse has been observed.
Isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Properdin factor B is a heat-labile serum protein active in the alternative complement activation pathway. It is binding to the C3b component of complement. The protein has a molecular weight of 92,000 and its mean concentration in human plasma is 200 μg/mL.Synonyms: BF, BFD, C3/C5 convertase, CFB, Glycine-rich beta glycoprotein, PBF2, Properdin factor B