|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||147 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies|
|Immunofluorescence (IF)||20 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||12 Antibodies|
|Immunohistochemistry (IHC)||43 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||11 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||41 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||60 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||1 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies|
|Antigen||Complement Component Receptor 1 (CD35) (CR1) Antibodies|
|Conjugate||This CR1 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|4 references available|
|Supplier||Log in to see|
Product Details anti-CR1 AntibodyTarget Details CR1 Application Details Handling References for anti-CR1 Antibody (ABIN111245) Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Immunogen||Purified C3b receptor from Human red blood cells.|
Target Details CR1Product Details anti-CR1 Antibody Application Details Handling References for anti-CR1 Antibody (ABIN111245) Images back to top
|Alternative Name||CD35 / C3BR (CR1 Antibody Abstract)|
|Background||This gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CR1 Antibody Target Details CR1 Handling References for anti-CR1 Antibody (ABIN111245) Images back to top
Mechanism of degradation of C3b and Analysis of Lymphoid and Myeloid differentiation: Clone J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3bdependent rosette formation with Lymphocytes. Fluorescence Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry: 1/25-1/50. Suitable on Frozen sections or cell smears, and onBouin's fixed, Paraffin-embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CR1 Antibody Target Details CR1 Application Details References for anti-CR1 Antibody (ABIN111245) Images back to top
|Reconstitution||Restore with 1 mL of distilled water.|
|Concentration||0.2 mg/mL (prior to lyophilization)|
|Buffer||PBS containing 1 mg/mL BSA as stabilizer and no preservative.|
|Handling Advice||Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.|
|Storage Comment||Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.|
References for anti-CR1 Antibody (ABIN111245)Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling Images back to top
|Product cited in:||
Schwartz, Barker, Long et al.: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, 2012 (PubMed).
Molnar: "Glycoproteins of Ehrlich ascites carcinoma cells. Incorporation of [14C]glucosamine and [14C]sialic acid into membrane proteins." in: Biochemistry, Vol. 6, Issue 10, pp. 3064-76 (PubMed).
Packer, Stevens, Cook et al.: "Diffusion of thrombolysis for acute myocardial infarction from 1981 to 2000 in England: trend analysis and comparison with need." in: International journal of technology assessment in health care, Vol. 20, Issue 4, pp. 531-6, 2004 (PubMed).
Cook, Fischer, Boucheix et al.: "Mouse monoclonal antibodies to the human C3b receptor." in: Molecular immunology, Vol. 22, Issue 5, pp. 531-9, 1985 (PubMed).