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anti-Human CR1 antibody for Functional Studies

Recommended CR1 Antibody (supplied by: Log in to see )

Antigen
Complement Component Receptor 1 (CD35) (CR1) Antibodies
  • C3BR
  • C3DR
  • C4BR
  • CD21
  • CD35
  • Cr-1
  • Cr-2
  • Cr1
  • KN
Reactivity
Human
293
61
46
46
41
10
10
5
2
Host
Mouse
264
71
2
Clonality
Monoclonal
Conjugate
This CR1 antibody is un-conjugated
37
36
16
8
8
7
7
7
6
6
6
6
6
6
6
6
6
6
3
2
2
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
280
179
131
124
85
68
67
63
35
11
11
4
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN111245
$ 610.50
Plus shipping costs $45.00

Top referenced anti-CR1 antibody for Functional Studies

Similar anti-CR1 Antibodies

Application / Reactivity Human
ELISA 35 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 239 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 63 Antibodies
Immunofluorescence (IF) 68 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 68 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 84 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 132 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunoprecipitation (IP) 80 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen Complement Component Receptor 1 (CD35) (CR1) Antibodies
Reactivity Human
(293), (61), (46), (46), (41), (10), (10), (5), (2)
Host Mouse
(264), (71), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CR1 antibody is un-conjugated
(37), (36), (16), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(280), (179), (131), (124), (85), (68), (67), (63), (35), (11), (11), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CR1 Antibody

Target Details CR1 Application Details Handling References for anti-CR1 Antibody (ABIN111245) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Immunogen Purified C3b receptor from Human red blood cells.
Clone J3D3
Isotype IgG1

Target Details CR1

Product Details anti-CR1 Antibody Application Details Handling References for anti-CR1 Antibody (ABIN111245) Images back to top
Antigen
Alternative Name CD35 / C3BR (CR1 Antibody Abstract)
Background This gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1
Gene ID 9606
UniProt P17927
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CR1 Antibody Target Details CR1 Handling References for anti-CR1 Antibody (ABIN111245) Images back to top
Application Notes Mechanism of degradation of C3b and Analysis of Lymphoid and Myeloid differentiation: Clone J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3bdependent rosette formation with Lymphocytes. Fluorescence Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry: 1/25-1/50. Suitable on Frozen sections or cell smears, and onBouin's fixed, Paraffin-embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CR1 Antibody Target Details CR1 Application Details References for anti-CR1 Antibody (ABIN111245) Images back to top
Reconstitution Restore with 1 mL of distilled water.
Concentration 0.2 mg/mL (prior to lyophilization)
Buffer PBS containing 1 mg/mL BSA as stabilizer and no preservative.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C
Storage Comment Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.

References for anti-CR1 Antibody (ABIN111245)

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling Images back to top
Product cited in:

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, 2012

Molnar: "Glycoproteins of Ehrlich ascites carcinoma cells. Incorporation of [14C]glucosamine and [14C]sialic acid into membrane proteins." in: Biochemistry, Vol. 6, Issue 10, pp. 3064-76, 1970

Background publications

Packer, Stevens, Cook, Raftery: "Diffusion of thrombolysis for acute myocardial infarction from 1981 to 2000 in England: trend analysis and comparison with need." in: International journal of technology assessment in health care, Vol. 20, Issue 4, pp. 531-6, 2004

Cook, Fischer, Boucheix, Mirsrahi, Jouvin, Weiss, Jack, Kazatchkine: "Mouse monoclonal antibodies to the human C3b receptor." in: Molecular immunology, Vol. 22, Issue 5, pp. 531-9, 1985