CD35 antibody

Catalog No. ABIN962071

This Mouse Monoclonal antibody specifically detects CD35 in IHC, FACS, WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Baboon, Cynomolgus and Rhesus Monkey.

Quick Overview for CD35 antibody (ABIN962071)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Baboon, Cynomolgus, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: E11

Product Details anti-CD35 Antibody

Specificity: Recognizes CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.

Purification: Protein A purified

Immunogen: Human acute monocytic leukaemia cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:50 - 1:200), IHC, IHC-Fr (1 - 10 μg/mL), IHC-P (10 μg/mL), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CR1 / CD35

Background: Name/Gene ID: CR1
Subfamily: Serine S1
Family: Protease

Synonyms: CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity