anti-Human CR1 antibody for Immunohistochemistry

Recommended CR1 Antibody (supplied by: Log in to see )

Antigen
Complement Component Receptor 1 (CD35) (CR1) Antibodies
  • C3BR
  • C4BR
  • CD35
  • KN
  • C3DR
  • CD21
  • Cr-1
  • Cr-2
  • Cr1
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • complement component (3d/Epstein Barr virus) receptor 2
  • complement receptor 2
  • complement receptor type 1-like
  • CR1
  • CR2
  • Cr2
  • LOC100514723
Reactivity
Baboon, Cynomolgus, Human, Rhesus Monkey
308
69
51
47
46
16
14
3
2
Host
Mouse
272
78
2
Clonality
Monoclonal
Conjugate
This CR1 antibody is un-conjugated
35
34
15
10
10
9
9
7
7
6
6
6
6
6
6
6
6
4
4
4
4
3
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
268
160
122
104
67
67
49
47
40
13
11
9
6
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN3026589
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.452184 ABIN2466920 FACS IHC Mouse IgG1 Log in to see E11
9.452184 ABIN4292461 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
9.452184 ABIN3025802 FACS IF IHC Mouse IgG1, kappa Log in to see SPM554
9.452184 ABIN2432879 IHC ELISA Rabbit IgG Log in to see Polyclonal
9.452184 ABIN2434494 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN4292473 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E11 4
1 ABIN135765 FACS IHC (p) IP IHC WB Mouse IgG1 Log in to see E11 2
1 ABIN2225643 FACS IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see E11
1 ABIN4292463 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN4292462 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN135767 FACS IHC (p) IHC PE Mouse IgG1 Log in to see E11 1
1 ABIN135766 FACS IHC (p) IHC FITC Mouse IgG1 Log in to see E11 1
1 ABIN1820989 IHC Mouse IgG2b Log in to see RLB25
1 ABIN3026590 IHC Mouse IgG1, kappa Log in to see E11
1 ABIN4292471 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E11
1 ABIN4292472 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E11
1 ABIN205999 FACS IHC FITC Mouse IgG1 Log in to see
1 ABIN4292490 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see E11
1 ABIN2471700 FACS IHC WB Mouse IgG1 Log in to see E11
1 ABIN3199787 FACS ICC IHC IP WB FITC Mouse IgG1 Log in to see E11

General

Antigen Complement Component Receptor 1 (CD35) (CR1) Antibodies
Reactivity Baboon, Cynomolgus, Human, Rhesus Monkey
(308), (69), (51), (47), (46), (16), (14), (3), (2)
Host Mouse
(272), (78), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CR1 antibody is un-conjugated
(35), (34), (15), (10), (10), (9), (9), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(268), (160), (122), (104), (67), (67), (49), (47), (40), (13), (11), (9), (6), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-CR1 Antibody

Target Details CR1 Application Details Handling Images
Characteristics This antibody recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
Purification Protein G affinity chromatography
Immunogen Intact human monocytes were used as the immunogen for this CD35 antibody.
Clone E11
Isotype IgG1, kappa

Target Details CR1

Product Details anti-CR1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD35 (CR1 Antibody Abstract)
Background This antibody recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
Gene ID 1378
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-CR1 Antibody Target Details CR1 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-CR1 Antibody Target Details CR1 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Complement Component Receptor 1 (CD35) (CR1) antibody (ABIN3026589) IHC testing of human tonsil stained with CD35 antibody (E11). Note cell membrane stai...