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Human Kallikrein 1 ELISA Kit

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Antigen
Kallikrein 1 (KLK1) ELISA Kits
  • 0610007D04Rik
  • hK1
  • KAL
  • Kal
  • kallikrein
  • kallistatin
  • Klk1b3
  • Klk1b6
  • Klk1c1
  • Klk6
  • KLKR
  • klkr
  • KLST
  • KST
  • mGk-6
  • Ngfg
  • PI-4
  • PI4
  • rGK-1
  • rK-1
Epitope
AA 25-262
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
8
6
2
2
2
2
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921120
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32682037 ABIN419080 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 6.25-400 ng/mL 6.25 ng/mL Log in to see
0.32682037 ABIN1570871 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see
0.32682037 ABIN2708298 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.32682037 ABIN2114379 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (EDTA - heparin) Log in to see
0.0012100277 ABIN1873413 Competition ELISA Serum, Plasma, Biological Fluids 74.07-6000 pg/mL 74.07 pg/mL Log in to see 1
0.0012100277 ABIN366179 Sandwich ELISA 0.78-50 ng/mL 0.78 ng/mL Log in to see
0.0012100277 ABIN510996 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 5-100 ng/mL 5 ng/mL Log in to see
0.0012100277 ABIN1884506 Sandwich ELISA 18.75-1200 pg/mL 18.75 pg/mL Log in to see
0.0012100277 ABIN1029057 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 9.375-600 pg/mL 9.375 pg/mL Log in to see
0.0012100277 ABIN2042571 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.0012100277 ABIN2684803 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Cell Lysate, Serum, Tissue Lysate 156-10000 pg/mL 156 pg/mL Log in to see
0.0012100277 ABIN2043837 Competition ELISA 123.46-10000 pg/mL 123.46 pg/mL Log in to see
0.0012100277 ABIN2535739 Competition ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 74.07-6000 pg/mL 74.07 pg/mL Log in to see
0.0012100277 ABIN455642 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012100277 ABIN2089341 Sandwich ELISA Log in to see
0.0012100277 ABIN2116175 156-10000 pg/mL 156 pg/mL Log in to see
0.0012100277 ABIN2948951 0.312-20 ng/mL 0.312 ng/mL Log in to see

Top referenced Kallikrein 1 ELISA Kit for ELISA

Similar Kallikrein 1 ELISA Kits

Application / Reactivity Human
ELISA 18 ELISA Kits

General

Antigen Kallikrein 1 (KLK1) ELISA Kits
Epitope AA 25-262
Reactivity Human
Kits with alternative reactivity to:
(17), (8), (6), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details Kallikrein 1 ELISA Kit

Target details Application Details Handling References for Kallikrein 1 Kit (ABIN921120) Images
Purpose For quantitative detection of human KLK1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human KLK1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human KLK1 antibod - 1
  • Lyophilized recombinant human KLK1 standar - 10ng/tubex2
  • Biotinylated anti- human KLK1 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: I25-S262

Target details

Product Details Kallikrein 1 ELISA Kit Application Details Handling References for Kallikrein 1 Kit (ABIN921120) Images back to top
Antigen
Alternative Name KLK1 (KLK1 ELISA Kit Abstract)
Background Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Gene ID 3816
UniProt P06870
Pathways Complement System

Application Details

Product Details Kallikrein 1 ELISA Kit Target details Handling References for Kallikrein 1 Kit (ABIN921120) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human KLK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK1 has been precoated onto 96-well plates. Standards(NSO, I25-S262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 1468, Standard deviation: 67.53, CV(%): 4.6
  • Sample 2: n=16, Mean(pg/ml): 3882, Standard deviation: 260.1, CV(%): 6.7
  • Sample 3: n=16, Mean(pg/ml): 6352, Standard deviation: 349.4, CV(%): 5.5,
  • Sample 1: n=24, Mean(pg/ml): 1625, Standard deviation: 95.9, CV(%): 5.9
  • Sample 2: n=24, Mean(pg/ml): 4190, Standard deviation: 301.7, CV(%): 7.2
  • Sample 3: n=24, Mean(pg/ml): 6526, Standard deviation: 417.7, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details Kallikrein 1 ELISA Kit Target details Application Details References for Kallikrein 1 Kit (ABIN921120) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Kallikrein 1 Kit (ABIN921120)

Product Details Kallikrein 1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Lundwall, Band, Blaber, Clements, Courty, Diamandis, Fritz, Lilja, Malm, Maltais, Olsson, Petraki, Scorilas, Sotiropoulou, Stenman, Stephan, Talieri, Yousef: "A comprehensive nomenclature for serine proteases with homology to tissue kallikreins." in: Biological chemistry, Vol. 387, Issue 6, pp. 637-41, 2006

Richards, Holman, Shen, Kozman, Harley, Brook, Shaw: "Human glandular Kallikrein genes: genetic and physical mapping of the KLK1 locus using a highly polymorphic microsatellite PCR marker." in: Genomics, Vol. 11, Issue 1, pp. 77-82, 1992

Images

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Supplier Images
ELISA image for Kallikrein 1 ELISA Kit (KLK1) (ABIN921120) Human KLK1 PicoKine ELISA Kit standard curve