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Human Kallikrein 14 ELISA Kit

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Antigen
Kallikrein 14 (KLK14) ELISA Kits
  • GK14
  • KLK-L6
Epitope
AA 19-248
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
8
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN1889328
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.31887746 ABIN419063 Competition ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 123.46-10000 pg/mL 123.46 pg/mL Log in to see
0.31887746 ABIN2708299 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012205833 ABIN842248 Sandwich ELISA Serum, Plasma, Tissue Homogenate 100-1.56 ng/mL 100 ng/mL Log in to see
0.0012205833 ABIN1570915 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 123.46-10000 pg/mL 123.46 pg/mL Log in to see
0.0012205833 ABIN2703282 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 1.5-500 pg/mL 1.5 pg/mL Log in to see
0.0012205833 ABIN2684804 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Cell Lysate, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012205833 ABIN2535753 Competition ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 123.46-10000 pg/mL 123.46 pg/mL Log in to see
0.0012205833 ABIN2948949 Log in to see

Top referenced Kallikrein 14 ELISA Kit for ELISA

Similar Kallikrein 14 ELISA Kits

Application / Reactivity Human
ELISA 9 ELISA Kits

General

Antigen Kallikrein 14 (KLK14) ELISA Kits
Epitope AA 19-248
Reactivity Human
Kits with alternative reactivity to:
(8), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details Kallikrein 14 ELISA Kit

Target details Application Details Handling References for Kallikrein 14 Kit (ABIN1889328) Images
Purpose For quantitative detection of human Kallikrein 14 in cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant human Kallikrein 14
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human Kallikrein 14 antibod - 1
  • Lyophilized recombinant human Kallikrein 14 standar - 10ng/tubex2
  • Biotinylated anti- human Kallikrein 14 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Q19-M248

Target details

Product Details Kallikrein 14 ELISA Kit Application Details Handling References for Kallikrein 14 Kit (ABIN1889328) Images back to top
Antigen
Alternative Name KLK14 (KLK14 ELISA Kit Abstract)
Background Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.
Gene ID 9622
UniProt Q9P0G3
Pathways Complement System

Application Details

Product Details Kallikrein 14 ELISA Kit Target details Handling References for Kallikrein 14 Kit (ABIN1889328) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Kallikrein 14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein 14 has been precoated onto 96-well plates. Standards(NSO, Q19-M248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein 14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein 14 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Kallikrein 14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallikrein 14 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 124, Standard deviation: 8.31, CV(%): 6.7
  • Sample 2: n=16, Mean(pg/ml): 364, Standard deviation: 17.5, CV(%): 4.8
  • Sample 3: n=16, Mean(pg/ml): 613, Standard deviation: 32.5, CV(%): 5.3,
  • Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 10.66, CV(%): 7.3
  • Sample 2: n=24, Mean(pg/ml): 408, Standard deviation: 22.44, CV(%): 5.5
  • Sample 3: n=24, Mean(pg/ml): 644, Standard deviation: 39.3, CV(%): 6.1
Restrictions For Research Use only

Handling

Product Details Kallikrein 14 ELISA Kit Target details Application Details References for Kallikrein 14 Kit (ABIN1889328) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Kallikrein 14 Kit (ABIN1889328)

Product Details Kallikrein 14 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Hooper, Bui, Rae, Harvey, Myers, Ashworth, Clements: "Identification and characterization of KLK14, a novel kallikrein serine protease gene located on human chromosome 19q13.4 and expressed in prostate and skeletal muscle." in: Genomics, Vol. 73, Issue 1, pp. 117-22, 2001

Images

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Supplier Images
ELISA image for Kallikrein 14 ELISA Kit (KLK14) (ABIN1889328) Human Kallikrein 14 PicoKine ELISA Kit standard curve