anti-Human PLG antibody for Radial Immunodiffusion

Recommended PLG Antibody (supplied by: Log in to see )

Antigen
Plasminogen (PLG) Antibodies
  • wu:fb70e09
  • PLG
  • LPA
  • plg
  • Ab1-346
  • AI649309
  • Pg
  • plasminogen
  • plasminogen-like
  • plg
  • PLG
  • Plg
  • LOC100725098
  • LOC100050531
Reactivity
Human
419
101
56
7
6
4
4
4
3
2
1
1
Host
Goat
218
165
49
42
25
2
Clonality
Polyclonal
Conjugate
This PLG antibody is un-conjugated
36
26
24
10
7
7
6
6
6
6
6
6
6
6
6
6
6
5
4
4
1
1
1
1
1
1
1
1
1
Application
Immunodiffusion (ID), Immunoelectrophoresis (IEP), Nephelometry (Neph), Radial Immunodiffusion (RID), ELISA
311
227
139
124
36
28
27
19
19
17
17
15
14
13
7
5
4
4
3
3
3
2
1
1
1
1
1
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Catalog No. ABIN457967
$ 229.09
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN458108 ID IEP Neph RID ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2476152 RID WB Rabbit IgG Log in to see Polyclonal
1 ABIN645144 RID WB Rabbit Log in to see

General

Antigen Plasminogen (PLG) Antibodies
Reactivity Human
(419), (101), (56), (7), (6), (4), (4), (4), (3), (2), (1), (1)
Host Goat
(218), (165), (49), (42), (25), (2)
Clonality
Polyclonal
Conjugate This PLG antibody is un-conjugated
(36), (26), (24), (10), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunodiffusion (ID), Immunoelectrophoresis (IEP), Nephelometry (Neph), Radial Immunodiffusion (RID), ELISA
(311), (227), (139), (124), (36), (28), (27), (19), (19), (17), (17), (15), (14), (13), (7), (5), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-PLG Antibody

Target Details PLG Application Details Handling Images
Specificity The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion ea reaction has been observed with bovine, dog and rhesus monkey.
Characteristics Precipitating polyclonal goat antiserum to human plasminogen
Purification Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Immunogen Native plasminogen is a single polypeptide chain, synthesized in the liver. Its molecular weight has been reported to be 81,000 and 92,000. Part of the molecule contains the active serine esterase of plasmin. On activation it is converted to two polypeptide chains linked by disulphide bridges. Three different abnormal molecular forms of plasminogen have been described. Normal adult plasma contains 10-20 mg/100 ml plasminogen. Different congenital molecular structure abnormalities associated with recurrent thrombosis have been described, but are extremely rare. In liver disease plasminogen activity may be reduced. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details PLG

Product Details anti-PLG Antibody Application Details Handling Images back to top
Antigen
Alternative Name Plasminogen (PLG Antibody Abstract)
Background The defined antibody reactivity is restricted to plasminogen as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. No precipitation is obtained with any other plasma protein or with plasminogen-deficient plasma. The antiserum may also react with plasmin conversion intermediates and with plasmin
Pathways Complement System

Application Details

Product Details anti-PLG Antibody Target Details PLG Handling Images back to top
Application Notes In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony), immunonephelometry, solid phase assays, as catching or detection antibody in ELISA. EDTA should be added to the plasma samples and all assay components to stabilize the proteins, to prevent activation of plasminogen in plasma samples epsilon-aminocaproic acid may be added.
Restrictions For Research Use only

Handling

Product Details anti-PLG Antibody Target Details PLG Application Details Images back to top
Format Lyophilized
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human plasma in agar-block immunodiffusion
Buffer Delipidated, heat inactivated, lyophilized, stable whole serum.
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.