Rat (Rattus) SERPING1 ELISA Kit

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Antigen
Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1) ELISA Kits
  • fb64f02
  • im:7159527
  • si:dkeyp-110g5.2
  • wu:fb60c09
  • wu:fb62a08
  • wu:fb64f02
  • LOC100136072
  • C1-INH
  • C1INH
  • C1nh
  • C1IN
  • C1NH
  • HAE1
  • HAE2
  • serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
  • C1 inhibitor
  • serine (or cysteine) peptidase inhibitor, clade G, member 1
  • serping1
  • LOC100136072
  • SERPING1
  • Serping1
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
19
12
10
3
2
2
2
1
1
1
1
1
Method Type
Competition ELISA
Detection Range
7.812-500 ng/mL
Minimum Detection Limit
7.812 ng/mL
Application
ELISA
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Catalog No. ABIN1568694
$ 891.06
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.357693 ABIN822550 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Bronchoalveolar Lavage, Urine, Tissue Homogenate 15.6-1000 ng/mL 15.6 ng/mL Log in to see
12.357693 ABIN1114301 Sandwich ELISA Serum, Plasma, Biological Fluids 7.813-500 ng/mL 7.813 ng/mL Log in to see
12.357693 ABIN4947466 Sandwich ELISA Plasma, Serum, Biological Fluids 6.25-400 ng/mL 6.25 ng/mL Log in to see
1 ABIN626808 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 25-500 ng/mL 25 ng/mL Log in to see
1 ABIN5520043 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 7.813-500 ng/mL 7.813 ng/mL Log in to see
1 ABIN4968231 Competition ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 7.812-500 ng/mL 7.812 ng/mL Log in to see
1 ABIN2543230 Competition ELISA Serum, Plasma, Biological Fluids 6.17-500 ng/mL 6.17 ng/mL Log in to see
1 ABIN5072829 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see
1 ABIN2946549 7.813-500 ng/mL 7.813 ng/mL Log in to see
1 ABIN1148261 Log in to see

General

Antigen Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(19), (12), (10), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Method Type Competition ELISA
Detection Range 7.812-500 ng/mL
Minimum Detection Limit 7.812 ng/mL
Application ELISA
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Product Details SERPING1 ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of C1INH in rat serum, plasma and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 2.26 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target details

Product Details SERPING1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name C1INH (SERPING1 ELISA Kit Abstract)
Background Synonyms: C1 esterase inhibitor,C1 Inh,C1Inh,C1-inhibiting factor,Plasma protease C1 inhibitor,Serpin G1,Serping1
Gene ID 295703
UniProt Q6P734
Pathways Complement System

Application Details

Product Details SERPING1 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Assay Procedure This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

Product Details SERPING1 ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months

Images

Product Details SERPING1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1) ELISA Kit (ABIN1568694) Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1) ELISA Kit