CD21 antibody (AA 142-240)
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- Target See all CD21 (CR2) Antibodies
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
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Binding Specificity
- AA 142-240
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD21 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)
- Specificity
- Recognizes a protein of 140 kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary)
- Clone
- CR2-2754
- Isotype
- IgG1 kappa
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- Application Notes
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Positive Control: MOLT4, Raji or WEHI-231 cells. Tonsil.
Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
- Alternative Name
- CR2 (CR2 Products)
- Synonyms
- C3DR antibody, CD21 antibody, CR antibody, CVID7 antibody, SLEB9 antibody, Cd21 antibody, cd21 antibody, CR1 antibody, CR1-L antibody, CR2 antibody, complement C3d receptor 2 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, CR2 antibody, Cr2 antibody, CR1 antibody
- Molecular Weight
- 140kDa
- Gene ID
- 1380
- UniProt
- P20023
- Pathways
- Complement System
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