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EPO antibody (AA 101-193)

Name: EPO antibody (AA 101-193)
SKU: ABIN679718
Price: 384.62 USD
Availability: InStock

EPO Reactivity: Human, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro), DB Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-EPO Polyclonal Antibody, Unconjugated (ABIN679718) at 1:200 followed by conjugation to the secondary antibody and DAB staining (EPO antibody (AA 101-193))

IHC

Rat lung lysates probed (ABIN679718) at 1:200 in 4 °C. (EPO antibody (AA 101-193))

SDS

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with EPO Polyclonal Antibody, Unconjugated ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (EPO antibody (AA 101-193))

IHC (p)

3 images

(4 references)

Catalog No. ABIN679718

Datasheet as PDF

Target See all EPO Antibodies

EPO (Erythropoietin (EPO))
Binding Specificity
25

15

11

11

11

9

7

5

5

4

4

3

3

3

3

3

2

2

1

1

1

1

1

1
AA 101-193
Reactivity
121

30

23

7

6

4

4

4

2

1

1

1
Human, Rat
Host
106

48

2

1

1
Rabbit
Compatible Secondaries
Clonality
108

50
Polyclonal
Conjugate
87

17

13

8

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This EPO antibody is un-conjugated
Application
115

72

48

39

29

14

13

13

12

12

8

8

4

4

4

3

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Cross-Reactivity

Human, Rat

Predicted Reactivity

Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Erythropoietin

Isotype

IgG

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anti-Erythropoietin (EPO) (AA 20-48) antibody
EPO Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

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EPO Reactivity: Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Erythropoietin (EPO) (AA 28-191) antibody
EPO Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Erythropoietin (EPO) antibody
EPO Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 4F11 unconjugated

anti-Erythropoietin (EPO) (AA 28-193) antibody
EPO Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4G7 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
dot-blot()

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Desharnais, Naud, Ayotte: "Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system." in: Drug testing and analysis, Vol. 10, Issue 11-12, pp. 1698-1707, (2019) (PubMed).

Ichii, Nakamura, Irie, Kouguchi, Nakamura, Nakamura, Sato, Yokoyama, Horino, Sunden, Elewa, Kon: "Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes." in: Histochemistry and cell biology, Vol. 146, Issue 3, pp. 351-62, (2016) (PubMed).

Li, Chen, Shao, Tang, Chen, Chen, Xu: "Oxidative stress induces the decline of brain EPO expression in aging rats." in: Experimental gerontology, Vol. 83, pp. 89-93, (2016) (PubMed).

DeNiro, Al-Mohanna: "Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment." in: PLoS ONE, Vol. 9, Issue 7, pp. e101602, (2014) (PubMed).
Target

EPO (Erythropoietin (EPO))

Alternative Name

EPO (EPO Products)

Synonyms

EPO antibody, EP antibody, MVCD2 antibody, erythropoietin antibody, erythropoietin S homeolog antibody, erythropoietin a antibody, EPO antibody, epo antibody, epo.S antibody, Epo antibody, epoa antibody

Target Type

Hormone

Background

Synonyms: EP, MVCD2, Erythropoietin, EPO
Background: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Gene ID

2056

UniProt

P01588

Pathways

JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity