CSF2RA Protein (AA 23-320) (His tag)
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- Target See all CSF2RA Proteins
- CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-320
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CSF2RA protein is labelled with His tag.
- Purpose
- Human GM-CSF R alpha Protein
- Sequence
- Glu23-Gly320
- Characteristics
- Recombinant Human GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu23-Gly320.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GM-CSF, No Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, His Tag with the EC50 of 1.0μg/ml determined by ELISA. The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 23-320) protein (Fc Tag)
CSF2RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) protein (His-Avi Tag,Biotin)CSF2RA Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 20-317) protein (His tag)CSF2RA Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 30-327) protein (His tag)CSF2RA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 1-400) protein (GST tag)CSF2RA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (Active) protein (Fc Tag)CSF2RA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 23-320) protein (His tag)Crystallography grade CSF2RA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 30-327) protein (His tag)Crystallography grade CSF2RA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 23-400) protein (rho-1D4 tag)Crystallography grade CSF2RA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 30-388) protein (rho-1D4 tag)Crystallography grade CSF2RA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
- Alternative Name
- GM-CSF R alpha (CSF2RA Products)
- Synonyms
- CD116 Protein, CDw116 Protein, CSF2R Protein, CSF2RAX Protein, CSF2RAY Protein, CSF2RX Protein, CSF2RY Protein, GM-CSF-R-alpha Protein, GMCSFR Protein, GMR Protein, SMDP4 Protein, Csfgmra Protein, GM-CSF-Ra Protein, GM-CSFR Protein, colony stimulating factor 2 receptor, alpha Protein, colony stimulating factor 2 receptor alpha subunit Protein, colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) Protein, CSF2RA Protein, Csf2ra Protein
- Background
- In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
- Molecular Weight
- 35.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling
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