FGF21 Protein (mFc-Avi Tag)
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- Target See all FGF21 Proteins
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FGF21 protein is labelled with mFc-Avi Tag.
- Sequence
- His29-Ser209
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (His tag)
FGF21 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (mFc-Avi Tag,Biotin)FGF21 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (His tag)FGF21 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-210) protein (His tag)FGF21 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (Active) proteinFGF21 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 21 (FGF21) (AA 29-208) protein (His tag)FGF21 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 21 (FGF21) (AA 30-209) protein (GST tag)FGF21 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (His tag)Crystallography grade FGF21 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- Alternative Name
- FGF21 (FGF21 Products)
- Synonyms
- FGF21 Protein, fibroblast growth factor 21 Protein, FGF21 Protein, fgf21 Protein, Fgf21 Protein
- Background
- UNQ3115, PRO10196, FGF-21, FGF21,Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
- Molecular Weight
- 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
- Pathways
- RTK Signaling
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