P2RX4 antibody (C-Term)
-
- Target See all P2RX4 Antibodies
- P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))
-
Binding Specificity
- C-Term
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This P2RX4 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- Purpose
- P2X4 / P2X4R
- Sequence
- YREKKYKYVE DYEQ
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-YREKKYKYVEDYEQ, from the C Terminus of the protein sequence according to NP_002551.2.
- Isotype
- IgG
-
-
- Application Notes
-
Western Blot: Approx 60 kDa band observed in Human Kidney lysates and in preliminary testing of Human Liver lysate (calculated MW of 43.4 kDa according to NP_002551.2).This molecular weight is routinely observed by other sources and was successfully blocke
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
-
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 3, pp. 907-15, (2011) (PubMed).
: "Role of mast cell activation in inducing microglial cells to release neurotrophin." in: Journal of neuroscience research, Vol. 88, Issue 6, pp. 1348-54, (2010) (PubMed).
: "
-
Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 3, pp. 907-15, (2011) (PubMed).
-
- Target
- P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))
- Alternative Name
- P2RX4 (P2RX4 Products)
- Synonyms
- P2X4 antibody, P2X4R antibody, AI504491 antibody, AW555605 antibody, D5Ertd444e antibody, p2rx4 antibody, p2x4 antibody, wu:fi03f02 antibody, p2x4r antibody, p2rx4.2 antibody, p2xr4.2 antibody, purinergic receptor P2X 4 antibody, purinergic receptor P2X, ligand-gated ion channel 4 antibody, purinergic receptor P2X, ligand-gated ion channel, 4a antibody, purinergic receptor P2X, ligand gated ion channel, 4 L homeolog antibody, purinergic receptor P2X, ligand-gated ion channel, 4b antibody, P2RX4 antibody, P2rx4 antibody, p2rx4a antibody, p2rx4.L antibody, p2rx4b antibody
- Background
- P2X4, P2X4R, P2RX4, purinergic receptor P2X, ligand-gated ion channel, 4, ATP receptor, ATP-gated cation channel protein, P2X purinoceptor 4, P2X receptor, subunit 4, purinergic receptor P2X ligand-gated ion channel 4, purinergic receptor P2X4, purinocept
- Gene ID
- 5025, 18438, 29659
- NCBI Accession
- NP_002551
- Pathways
- Synaptic Membrane
-