For best experience we recommend to activate Javascript in your browser.

MYCN antibody (AA 401-464)

Name: MYCN antibody (AA 401-464)
SKU: ABIN760676
Price: 384.62 USD
Availability: InStock
Rating: 5 (1 reviews)

MYCN Reactivity: Human, Mouse ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 401-464) antibody (ABIN760676) (MYCN antibody (AA 401-464))

Image provided by the Independent Validation Program (badge number 29800). (MYCN antibody (AA 401-464))

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-n-Myc Polyclonal Antibody, Unconjugated (ABIN760676) at 1:200 followed by conjugation to the secondary antibody and DAB staining (MYCN antibody (AA 401-464))

IHC

Formalin-fixed and paraffin embedded human brain labeled with Anti-n-Myc Polyclonal Antibody, Unconjugated (ABIN760676) at 1:200 followed by conjugation to the secondary antibody and DAB staining (MYCN antibody (AA 401-464))

IHC

4 images

(1 reference)

(1 validation)

Catalog No. ABIN760676

Datasheet as PDF

Target See all MYCN Antibodies

MYCN (N-Myc Proto-Oncogene Protein (MYCN))
Binding Specificity
14

6

4

3

3

2

1

1

1

1

1

1
AA 401-464
Reactivity
45

31

14

4

3

2

2

2

1

1

1

1
Human, Mouse
Host
43

7
Rabbit
Compatible Secondaries
Clonality
45

5
Polyclonal
Conjugate
26

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MYCN antibody is un-conjugated
Application
24

14

12

12

6

4

3

2

1

1

1

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity

Rat,Cow,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human n-Myc

Isotype

IgG
anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 168-267) antibody
MYCN Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 337-464) antibody
MYCN Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 2A4E9 unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 1-100) antibody
MYCN Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 3H4 unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 322-351), (C-Term) antibody
MYCN Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB18771 unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) (Internal Region) antibody
MYCN Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) antibody
MYCN Reactivity: Human, Mouse, Rat ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 1-464) antibody
MYCN Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 1-100) antibody
MYCN Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4H4 unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) antibody
MYCN Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated

anti-N-Myc Proto-Oncogene Protein (MYCN) (C-Term) antibody
MYCN Reactivity: Human WB, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Validation #029800 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029800

Date

08/21/2014

Antigen

Lot Number

120917

Method validated

Western Blotting

Positive Control

MCF7 cells (variant MCF7/VP)00284-0/pdf)

Negative Control

SK-N-SH cells - RNA

Notes

A strong band was observed in the positive control sample at the correct molecular weight. An additional band was also observed in the positive sample at a higher molecular weight which may represent non-specific binding. No bands were observed in the negative control sample.

Validation Images

Figure 1: Western Blot for MYCN. Arrowhead indicates the expected molecular weight of ~49 kDa.
Full Methods
Primary Antibody

Antigen: Mycn (MYCN)
Catalog number: ABIN760676
Lot number: 120917
Antibody Dilution: 1:200

Secondary Antibody

Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Lot number: L170-6515
Antibody Dilution: 1:10,000

Full Protocol

1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 13 hours at 4°C.
8. The membrane was rinsed in TBST thrice for 5 minutes each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
11. The membrane was rinsed in TBS twice for 30 seconds each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
15. The membrane was washed in TBST 2 times for 10 minutes each.
16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.

Experimental Notes

- No experimental challenges noted.
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Ramraj, Aravindan, Somasundaram, Herman, Natarajan, Aravindan: "Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." in: Oncotarget, Vol. 7, Issue 14, pp. 18605-19, (2016) (PubMed).
Target

MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Alternative Name

N-Myc (MYCN Products)

Synonyms

MODED antibody, MYCNOT antibody, N-myc antibody, NMYC antibody, ODED antibody, bHLHe37 antibody, Nmyc antibody, Nmyc-1 antibody, Nmyc1 antibody, c-nmyc antibody, Nmuc1 antibody, fb57a02 antibody, nmyc1 antibody, wu:fb57a02 antibody, zgc:85706 antibody, XN-myc antibody, XNmyc antibody, mycn antibody, mycn-a antibody, mycn-b antibody, mycna antibody, xN-myc1 antibody, MYCN proto-oncogene, bHLH transcription factor antibody, v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody, v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived antibody, olfactory receptor 8S1 antibody, MYCN proto-oncogene, bHLH transcription factor S homeolog antibody, MYCN antibody, Mycn antibody, LOC515887 antibody, mycn antibody, mycn.S antibody

Background

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq, Jul 2008].
Subcellular location: Nucleus
Synonyms: NMYC, ODED, MODED, N-myc, bHLHe37, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, MYCN

Gene ID

4613

UniProt

P04198