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MYCN antibody (AA 401-464)

MYCN Reactivity: Human, Mouse ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN760676
  • Target See all MYCN Antibodies
    MYCN (N-Myc Proto-Oncogene Protein (MYCN))
    Binding Specificity
    • 14
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 401-464
    Reactivity
    • 45
    • 31
    • 14
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 43
    • 7
    Rabbit
    Clonality
    • 45
    • 5
    Polyclonal
    Conjugate
    • 26
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MYCN antibody is un-conjugated
    Application
    • 24
    • 14
    • 12
    • 12
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Predicted Reactivity
    Rat,Cow,Pig
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human n-Myc
    Isotype
    IgG
  • Application Notes
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029800 (Western Blotting)
    'Independent Validation' Badge
    by
    Alamo Laboratories Inc
    No.
    #029800
    Date
    08/21/2014
    Antigen
    Lot Number
    120917
    Method validated
    Western Blotting
    Positive Control
    MCF7 cells (variant MCF7/VP)00284-0/pdf)
    Negative Control
    SK-N-SH cells - RNA
    Notes
    A strong band was observed in the positive control sample at the correct molecular weight. An additional band was also observed in the positive sample at a higher molecular weight which may represent non-specific binding. No bands were observed in the negative control sample.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Mycn (MYCN)
    • Catalog number: ABIN760676
    • Lot number: 120917
    • Antibody Dilution: 1:200
    Secondary Antibody
    • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
    • Lot number: L170-6515
    • Antibody Dilution: 1:10,000
    Full Protocol
    • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
    • 2. 15 μl of heated culture-media were loaded and resolved on 8-16% SDS-polyacrylamide gel.
    • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
    • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
    • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
    • 6. The membrane was rinsed with TBST once.
    • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 13 hours at 4°C.
    • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
    • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
    • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
    • 11. The membrane was rinsed in TBS twice for 30 seconds each.
    • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 45 minutes.
    • 13. The membrane was rinsed three times TBST.
    • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
    • 15. The membrane was washed in TBST 2 times for 10 minutes each.
    • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
    Experimental Notes
    - No experimental challenges noted.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Ramraj, Aravindan, Somasundaram, Herman, Natarajan, Aravindan: "Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease." in: Oncotarget, Vol. 7, Issue 14, pp. 18605-19, (2016) (PubMed).

  • Target
    MYCN (N-Myc Proto-Oncogene Protein (MYCN))
    Alternative Name
    N-Myc (MYCN Products)
    Synonyms
    MODED antibody, MYCNOT antibody, N-myc antibody, NMYC antibody, ODED antibody, bHLHe37 antibody, Nmyc antibody, Nmyc-1 antibody, Nmyc1 antibody, c-nmyc antibody, Nmuc1 antibody, fb57a02 antibody, nmyc1 antibody, wu:fb57a02 antibody, zgc:85706 antibody, XN-myc antibody, XNmyc antibody, mycn antibody, mycn-a antibody, mycn-b antibody, mycna antibody, xN-myc1 antibody, MYCN proto-oncogene, bHLH transcription factor antibody, v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody, v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived antibody, olfactory receptor 8S1 antibody, MYCN proto-oncogene, bHLH transcription factor S homeolog antibody, MYCN antibody, Mycn antibody, LOC515887 antibody, mycn antibody, mycn.S antibody
    Background

    This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq, Jul 2008].

    Subcellular location: Nucleus

    Synonyms: NMYC, ODED, MODED, N-myc, bHLHe37, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, MYCN

    Gene ID
    4613
    UniProt
    P04198
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