Vitronectin antibody
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- Target See all Vitronectin (VTN) Antibodies
- Vitronectin (VTN)
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Reactivity
- Human, Cow, Dog, Goat, Horse, Cat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vitronectin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes human vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Species Crossreactivity: feline, canine, goat, bovine and to a lesser extent with equine. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding.
- Purification
- Affinity purified
- Immunogen
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Human vitronectin purified from plasma by heparin-affinity chromatography.
Type of Immunogen: Purified protein - Isotype
- IgG1 kappa
- Top Product
- Discover our top product VTN Primary Antibody
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- Application Notes
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Approved: ELISA (1:30000), IHC, IHC-P (2.5 μg/mL), WB (1:50)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. ELISA: 1:15000. Coat with vitronectin directly onto the microtiter well. Please note that cross-reactivity with species other than human has not been confirmed in IHC at this time. In the absence of testing data we cannot guarantee cross-reactivity with non-human species in IHC. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 500 mM sodium chloride, 15 mM sodium azide, 40 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Vitronectin (VTN)
- Alternative Name
- VTN / Vitronectin (VTN Products)
- Synonyms
- VTN antibody, DKFZp470F0511 antibody, LOC100228142 antibody, vtn antibody, zgc:112277 antibody, V75 antibody, VN antibody, VNT antibody, AI256434 antibody, Vn antibody, Aa1018 antibody, vitronectin antibody, vitronectin a antibody, vitronectin b antibody, vitronectin L homeolog antibody, VTN antibody, vtn antibody, vtna antibody, vtnb antibody, CpipJ_CPIJ016691 antibody, vtn.L antibody, Vtn antibody
- Background
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Name/Gene ID: VTN
Family: Matrix Protein
Synonyms: VTN, Epibolin, Serum-spreading factor, VNT, S-protein, Somatomedin B, V75, VN, Complement S-protein, Serum spreading factor, Vitronectin - Gene ID
- 7448
- UniProt
- P04004
- Pathways
- Autophagy, Smooth Muscle Cell Migration
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